Exorista civilis Rondani (Diptera:Tachinidae) is an excellent dominant parasitic enemy all over the world. But there has been a lack of research on the molecular regulation of diapause in E. civilis. To investigate the important diapause-associated genes and metabolic pathways in E. civilis, we can provide a theoretical basis for clarifying the molecular mechanism of diapause at the transcriptome level. The Illumina HiSeq. 2000 platform was used to perform transcriptome sequencing and bioinformatics analysis of the non-diapause and diapause pupae of E. civilis. 58,050 unigenes were successfully assembled, in which 4355 upregulated and 3158 downregulated unigenes were differentially expressed. Moreover, by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichments, 896 kinds of the differentially expressed genes were specifically analyzed and showed that diapause-associated genes were related to be involved in the pathways of cold resistance, amino acid metabolism, and energy metabolism. Furthermore, these upregulated five genes showed the same trends of expression patterns between quantitative real-time polymerase chain reaction and RNA-Seq. This study provides a theoretical basis for the further study of the diapausing molecular mechanisms of E. civilis.

中文翻译：

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墨西哥洋蛇（Exorista civilis Rondani）滞育相关基因的转录组学分析（双翅目：Ta科）

Exorista civilis Rondani（Diptera：Tachinidae）是全世界极好的寄生寄生虫。但是缺乏对大肠杆菌滞育的分子调控的研究。为了研究民用大肠杆菌中重要的滞育相关基因和代谢途径，我们可以为在转录组水平上阐明滞育的分子机制提供理论依据。Illumina HiSeq。2000平台用于对大肠埃希菌的非滞育和滞育p进行转录组测序和生物信息学分析。成功装配了58,050个单基因，其中差异表达了4355个上调和3158个下调的单基因。此外，通过《京都基因与基因组百科全书》（KEGG）途径富集，对896种差异表达基因进行了专门分析，结果表明滞育相关基因与耐寒性，氨基酸代谢和能量代谢途径有关。代谢。此外，这五个上调的基因在定量实时聚合酶链反应和RNA-Seq之间显示出相同的表达模式趋势。本研究为进一步深入研究民间大肠杆菌的滞育分子机制提供了理论依据。