Induction of diapause by clock proteins period and timeless via changes in PTTH and ecdysteroid titer in the sugar beet moth, Scrobipalpa ocellatella (Lepidoptera: Gelechiidae),Archives of Insect Biochemistry and Physiology

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Induction of diapause by clock proteins period and timeless via changes in PTTH and ecdysteroid titer in the sugar beet moth, Scrobipalpa ocellatella (Lepidoptera: Gelechiidae)
Archives of Insect Biochemistry and Physiology ( IF 1.5 ) Pub Date : 2021-04-16 , DOI: 10.1002/arch.21790
Fatemeh Ahmadi ₁ , Azam Mikani ₁ , Saeid Moharramipour ₁

Affiliation

The sugar beet moth, Scrobipalpa ocellatella (Boyd), one of the most severe sugar beet pests, causes quantitative and qualitative yield losses late in the autumn. Previously, it was shown that low temperature and short-day photoperiod together cause diapause induction in pupae. Here, the interaction of the critical elements of the diapause induction, including the period (PER), timeless (TIM), prothoracicotropic hormone (PTTH), and ecdysteroid titer, were investigated. Immunohistochemistry results showed that the number of period immunoreactivity (PER-ir) and TIM-ir cells in nondiapause pupae (NDP) was lower than in the brain of the diapause pupae (DP). Moreover, the number of PER-ir and TIM-ir cells in the protocerebrum and optic lobe (OL) of NDP was lower than DP. Moreover, lower PTTH content in the brain and hemolymph of DP was confirmed by competitive enzyme-linked immunosorbent assay. Enzyme immunoassay showed a lower 20-hydroxyecdysone (20E) titer in the hemolymph of the DP compared with the NDP. Within a short-day condition, PER and TIM titers increased in the brain leading to decreasing PTTH titers in the brain and hemolymph that caused decreasing 20E titer in the hemolymph, leading to the induction of diapause. This study suggests that PER and TIM could be one of the brain factors that play an essential role in regulating diapause in S. ocellatella.

中文翻译：

通过甜菜蛾Scrobipalpa ocellatella中的PTTH和蜕皮甾类滴度的变化，时钟蛋白周期性地诱导滞育，并具有永恒性（鳞翅目：天蛾科）

甜菜蛾，Scrobipalpa ocellatella（Boyd）是最严重的甜菜害虫之一，在秋季后期导致数量和质量上的产量损失。以前的研究表明，低温和短时间的光周期共同导致di的滞育诱导。在这里，研究了滞育诱导的关键元素的相互作用，包括周期（PER），永恒（TIM），促促促甲状腺激素（PTTH）和蜕皮甾类滴度。免疫组织化学结果显示，非滞育p（NDP）的定期免疫反应（PER-ir）和TIM-ir细胞的数量低于滞育p（DP）的大脑。此外，NDP的前脑和视叶（OL）中的PER-ir和TIM-ir细胞数量低于DP。此外，通过竞争性酶联免疫吸附试验证实了DP的脑和淋巴中的PTTH含量较低。酶免疫分析显示，与NDP相比，DP的血淋巴中的20-羟基蜕皮酮（20E）滴度较低。在短时间内，大脑中的PER和TIM滴度增加，导致大脑和淋巴中的PTTH滴度降低，从而导致淋巴中的20E滴度降低，从而导致滞育。这项研究表明，PER和TIM可能是在调节大鼠滞育方面发挥重要作用的脑因素之一。沙门氏菌。

更新日期：2021-05-26

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