Abstract

The influence of a pancreatic enzyme, lipase, on doxorubicin-loaded anionic liposomes, their complexes with spherical polycationic brushes, and ternary complexes with brushes and bovine serum albumin has been studied. The two-stage kinetics of the enzyme-induced disturbance of liposomal membrane integrity has been investigated using fluorescence spectroscopy. A 120-min induction period free of any serious damage to liposome membranes has been found for all enzyme-treated samples. This period is followed by rapid release of encapsulated doxorubicin. Enzymatic hydrolysis of the liposomes and their binary complexes with brushes has been found to proceed at the same rate, while a rather long resistance to the disturbance of liposome integrity has been observed for the ternary complexes. It has been assumed that albumin plays the role of a sterically screening agent, which hinders the enzymatic hydrolysis in multiliposomal complexes.

中文翻译：

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用白蛋白修饰多脂质体纳米容器作为增加其对酶水解的抗性的方法

摘要

研究了胰酶，脂肪酶对负载阿霉素的阴离子脂质体，它们与球形聚阳离子刷的配合物以及与刷和牛血清白蛋白的三元配合物的影响。已经使用荧光光谱法研究了酶诱导的脂质体膜完整性紊乱的两阶段动力学。对于所有酶处理的样品，已发现120分钟的诱导期对脂质体膜没有任何严重损害。在此期间之后，迅速释放出封装的阿霉素。已经发现脂质体和它们的二元复合物用刷子的酶水解以相同的速率进行，而对于三元复合物，观察到了对脂质体完整性干扰的相当长的抵抗力。