Solanesol, a naturally occurring constituent of tobacco, has been utilized as a good marker for environmental tobacco smoke particulate and as a noninvasive predictor of mainstream cigarette smoke tar and nicotine intake under naturalistic smoking conditions. A fast and accurate method for measuring free solanesol to assess tobacco smoke exposure is highly desirable. We have developed and validated a new environmentally friendly, high-throughput method for measuring solanesol content in discarded cigarette filter butts. The solanesol deposited in the used filters can be correlated with mainstream smoke deliveries of nicotine and total particle matter to estimate constituent delivery to smokers. A portion of filter material is removed from cigarette butts after machine smoking, spiked with internal standard solution, extracted and quantitatively analyzed using reverse-phase liquid chromatography coupled to a triple-quadrupole mass spectrometer. The new method incorporates a 48-well plate format for automated sample preparation that reduces sample preparation time and solvent use and increases sample throughput 10-fold compared to our previous method. Accuracy and precision were evaluated by spiking known amounts of solanesol on both clean and smoked cigarette butts. Recoveries exceeded 93% at both low and high spiking levels. Linear solanesol calibration curves ranged from 1.9 to 367 µg/butt with a 0.05 µg/butt limit of detection.

中文翻译：

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液相色谱-质谱法测定香烟过滤嘴中的游离茄尼醇水平


茄尼醇是烟草中天然存在的成分，已被用作环境烟草烟雾颗粒的良好标记物，并作为自然吸烟条件下主流香烟烟雾焦油和尼古丁摄入量的无创预测因子。非常需要一种快速准确的方法来测量游离茄尼醇以评估烟草烟雾暴露。我们开发并验证了一种新的环保、高通量方法，用于测量废弃香烟滤嘴中的茄尼醇含量。沉积在所用过滤器中的茄尼醇可以与主流烟雾中尼古丁和总颗粒物的输送量相关联，以估计向吸烟者输送的成分。机器吸烟后从烟头中取出一部分过滤材料，加入内标溶液，提取并使用反相液相色谱与三重四极杆质谱仪联用进行定量分析。新方法采用 48 孔板格式进行自动样品制备，与我们之前的方法相比，减少了样品制备时间和溶剂使用量，并将样品通量提高了 10 倍。通过在干净的烟蒂和吸过的烟蒂上添加已知量的茄尼醇来评估准确度和精密度。在低加标水平和高加标水平下回收率均超过 93%。线性茄尼醇校准曲线范围为 1.9 至 367 µg/干基，检测限为 0.05 µg/干基。