This paper reports a smart intracellular nanocarrier for sustainable and controlled drug release in non-invasive neuroregeneration. The nanocarrier is composed by superparamagnetic iron oxide-gold (SPIO-Au) core-shell nanoparticles (NPs) conjugated with porous coordination cages (PCCs) through the thiol-containing molecules as bridges. The negatively charged PCC-2 and positively charged PCC-3 are compared for intracellular targeting. Both types result in intracellular targeting via direct penetration across cellular membranes. However, the pyrene (Py)-PEG-SH bridge enabled functionalization of SPIO-Au NPs with PCC-3 exhibits higher interaction with PC-12 neuron-like cells, compared with the rhodamine B (RhB)-PEG-SH bridge enabled case and the stand-alone SPIO-Au NPs. With neglectable toxicities to PC-12 cells, the proposed SPIO-Au-RhB(Py)-PCC-2(3) nanocarriers exhibit effective drug loading capacity of retinoic acid (RA) at 13.505 μg/mg of RA/NPs within 24 h. A controlled release of RA is achieved by using a low-intensity 525 nm LED light (100% compared to 40% for control group within 96 h).

本文报道了一种智能细胞内纳米载体，用于在非侵入性神经再生中实现可持续和可控的药物释放。纳米载体由超顺磁性氧化铁-金 (SPIO-Au) 核壳纳米粒子 (NPs) 通过含硫醇分子作为桥与多孔配位笼 (PCCs) 共轭组成。比较带负电的 PCC-2 和带正电的 PCC-3 的细胞内靶向。两种类型都通过直接穿透细胞膜导致细胞内靶向。然而，与启用罗丹明 B (RhB)-PEG-SH 桥的情况相比，芘 (Py)-PEG-SH 桥使 SPIO-Au NP 与 PCC-3 的功能化表现出与 PC-12 神经元样细胞更高的相互作用和独立的 SPIO-Au NP。对 PC-12 细胞具有可忽略的毒性，所提出的 SPIO-Au-RhB(Py)-PCC-2(3) 纳米载体在 24 小时内表现出 13.505 μg/mg RA/NPs 的视黄酸 (RA) 有效载药能力。通过使用低强度 525 nm LED 灯实现 RA 的受控释放（100% 相比对照组在 96 小时内为 40%）。