Abstract

The kinetics of interaction between glutathione (GSH) and unsaturated phenol resveratrol (RVT) in deionized water in the presence of hydrogen peroxide (H₂O₂) is studied. At a physiological concentration (0.1–10 mM), GSH containing two carboxyl groups forms acidic solutions (pH of 3–4); the GSH molecules are associated into dimers. Under these conditions, GSH is quite slowly oxidized by atmospheric oxygen, and the reaction between GSH and H₂O₂ is accompanied by the formation of radicals. The thiyl radical initiation rate (W_i) is a few fractions of a percent of the GSH consumption rate; however, it is sufficient to initiate a thiol–ene chain reaction between GSH and RVT. Using the experimental data on the kinetics and the product composition and the published data on reactions of GSH with H₂O₂ and thiyl radicals, a kinetic model of the complex interaction between GSH and RVT in the presence of H₂O₂ in an aqueous medium at 37°C is proposed. The model includes 19 quasi-elementary reactions with respective rate constants, in particular, the formation of intermediate GSH–H₂O₂ and GSH–GSH complexes, the formation of radicals, and their subsequent transformations into final products in reactions with RVT and GSH. A computer simulation based on the developed model adequately describes the features of the process kinetics in a wide reactant concentration range.

中文翻译：

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过氧化氢存在下谷胱甘肽与白藜芦醇的相互作用：动力学模型

摘要

研究了过氧化氢（H ₂ O ₂）存在下去离子水中谷胱甘肽（GSH）和不饱和酚白藜芦醇（RVT）之间相互作用的动力学。在生理浓度（0.1–10 mM）下，含有两个羧基的GSH形成酸性溶液（pH值为3-4）。GSH分子缔合成二聚体。在这些条件下，GSH相当缓慢地被大气中的氧气氧化，并且GSH与H ₂ O ₂之间的反应伴随有自由基的形成。巯基自由基引发速率（W _i）是GSH消耗率的百分之几；然而，足以在GSH和RVT之间引发硫醇-烯链反应。使用谷胱甘肽的反应在动力学实验数据和产品组合物和公布的数据用H ₂ ö ₂和含硫基团，GSH和RVT之间复杂的相互作用的在H存在下动力学模型₂ ö ₂在水性建议在37°C下使用培养基。该模型包括19个准元素反应，具有各自的速率常数，特别是中间GSH–H ₂ O _2的形成和GSH-GSH配合物，自由基的形成以及它们在与RVT和GSH的反应中转化为最终产物。基于已开发模型的计算机模拟充分描述了反应物在较宽的浓度范围内的过程动力学特征。