Mink astrovirus infection remains a poorly understood disease entity, and the aetiological agent itself causes disease with a heterogeneous course, including gastrointestinal and neurological symptoms. This paper presents cases of astrovirus infection in mink from continental Europe. RNA was isolated from the brains and intestines of animals showing symptoms typical of shaking mink syndrome (n = 6). RT-PCR was used to amplify astrovirus genetic material, and the reaction products were separated on a 1% agarose gel. The specificity of the reaction was confirmed by sequencing fragment coding RdRP protein (length of sequencing product 170 bp) from all samples. The presence of astrovirus RNA was detected in each of the samples tested. Sequencing and bioinformatic analysis indicated the presence of the same variant of the virus in all samples. Comparison of the variant with the sequences available in bioinformatics databases confirmed that the Polish isolates form a separate clade, closely related to Danish isolates. The dissimilarity of the Polish variant to those isolated in other countries ranged from 2.4% (in relation to Danish isolates) to 7.1% (in relation to Canadian isolates). Phylogenetic relationships between variants appear to be associated with the geographic distances between them. To our knowledge, this work describes the first results on the molecular epidemiology of MAstV in continental Europe. The detection of MAstV in Central Europe indicates the need for further research to broaden our understanding of the molecular epidemiology of MAstV in Europe.

水貂星状病毒感染仍然是一个知之甚少的疾病实体，病原体本身导致具有异质病程的疾病，包括胃肠道和神经系统症状。本文介绍了欧洲大陆水貂感染星状病毒的案例。RNA 是从动物的大脑和肠道中分离出来的，这些动物表现出典型的抖貂综合症（n = 6）。RT-PCR用于扩增星状病毒遗传物质，反应产物在1%琼脂糖凝胶上分离。反应的特异性通过对所有样品的编码 RdRP 蛋白的序列片段（测序产物长度 170 bp）进行确认。在每个测试的样品中都检测到星状病毒 RNA 的存在。测序和生物信息学分析表明所有样本中都存在相同的病毒变体。将该变体与生物信息学数据库中可用的序列进行比较，证实波兰分离株形成一个单独的进化枝，与丹麦分离株密切相关。波兰变异与在其他国家分离的变异的差异范围从 2.4%（相对于丹麦分离株）到 7.1%（相对于加拿大分离株）。变体之间的系统发育关系似乎与它们之间的地理距离有关。据我们所知，这项工作描述了欧洲大陆 MAstV 分子流行病学的第一个结果。在中欧检测到 MAstV 表明需要进一步研究以拓宽我们对欧洲 MAstV 分子流行病学的理解。