Abstract

Purpose

To examine the ablation zone, muscle contractions, and temperature increases in both rabbit liver and kidney models in vivo for a custom-made high-frequency irreversible electroporation (H-FIRE) generator.

Materials and Methods

A total of 18 New Zealand white rabbits were used to investigate five H-FIRE protocols (n = 3 for each protocol) and an IRE protocol (n = 3) for the performance of the designed H-FIRE device in both liver and kidney tissues. The ablation zone was determined by using histological analysis 72 h after treatment. The extent of muscle contractions and temperature change during the application of pulse energy were measured by a commercial accelerometer attached to animals and fiber optic temperature probe inserted into organs with IRE electrodes, respectively.

Results

All H-FIRE protocols were able to generate visible ablation zones without muscle contractions, for both liver and kidney tissues. The area of ablation zone generated in H-FIRE pulse protocols (e.g., 0.3–1 μs, 2000 V, and 90–195 bursts) appears similar to that of IRE protocol (100 μs, 1000 V, and 90 pulses) in both liver and kidney tissues. No significant temperature increase was noticed except for the protocol with the highest pulse energy (e.g., 1 μs, 2000 V, and 180 bursts).

Conclusion

Our work serves to complement the current H-FIRE pulse waveforms, which can be optimized to significantly improve the quality of ablation zone in terms of precision for liver and kidney tumors in clinical setting.

中文翻译：

---

定制的高频不可逆电穿孔发生器在临床上相关消融区域的不同组织上的体内研究

摘要

目的

为了检查定制高频不可逆电穿孔（H-FIRE）发生器在体内的兔子肝脏和肾脏模型中的消融区，肌肉收缩和温度升高。

材料和方法

总共18只新西兰白兔用于研究五种H-FIRE方案（ 每个方案n = 3）和IRE方案（n  = 3）以确保设计的H-FIRE设备在肝脏和肾脏组织中的性能。治疗后72小时通过组织学分析确定消融区。施加脉冲能量期间的肌肉收缩程度和温度变化通过分别连接到动物的商业加速度计和通过IRE电极插入器官的光纤温度探头进行测量。

结果

对于肝脏和肾脏组织，所有H-FIRE方案都能产生可见的消融区，而无肌肉收缩。在两个肝脏中，H-FIRE脉冲协议（例如0.3–1μs，2000 V和90–195脉冲）产生的消融区面积看起来类似于IRE协议（100μs，1000 V和90脉冲）和肾脏组织。除了具有最高脉冲能量（例如1μs，2000 V和180猝发）的协议外，没有发现明显的温度升高。

结论

我们的工作是对当前的H-FIRE脉冲波形进行补充，可以对这些波形进行优化，从而在临床环境中就肝脏和肾脏肿瘤的精确度而言显着改善消融区的质量。