In this study, the potential of certain lactic acid bacteria—classified as probiotics and known to be antimicrobially active against pathogens or food-poisoning microorganisms—was evaluated with respect to their activity against bacterial skin pathogens. The aim of the study was to develop a plaster/bandage for the application of inhibitory substances produced by these probiotics when applied to diseased skin. For this purpose, two Streptococcus salivarius strains and one Lactobacillus plantarum were tested for production of antimicrobials (bacteriocin-like substances) active against Gram-positive and Gram-negative pathogens using established methods. A newly designed membrane test ensured that the probiotics produce antimicrobials diffusible through membranes. Target organisms used were Cutibacterium acnes, Staphylococcus aureus, and Pseudomonas aeruginosa. Moreover, the L. plantarum 8P-A3 strain was tested against additional bacteria involved in skin disorders. The Lactobacillales used were active against all potential skin pathogens tested. These probiotics could be enclosed between polymer membranes—one tight, the other permeable for their products, preserved by vacuum drying, and reactivated after at least three months storage. Importantly, the reactivated pads containing the probiotics demonstrated antibacterial activity on agar plates against all pathogens tested. This suggests that the probiotic containing pads may be topically applied for the treatment of skin disorders without the need for a regular antibiotic treatment or as an adjunctive therapy.

在这项研究中，评估了某些乳酸菌（被归类为益生菌并已知对病原体或食物中毒微生物具有抗菌活性）的潜力，以评估它们对细菌性皮肤病原体的活性。该研究的目的是开发一种膏药/绷带，用于将这些益生菌产生的抑制物质应用于患病皮肤。为此目的，两种唾液链球菌菌株和一种植物乳杆菌使用已建立的方法测试了对革兰氏阳性和革兰氏阴性病原体有活性的抗菌剂（细菌素样物质）的生产。一项新设计的膜测试确保益生菌产生可通过膜扩散的抗菌剂。使用的目标生物是痤疮皮肤杆菌、金黄色葡萄球菌和铜绿假单胞菌。此外，植物乳杆菌8P-A3 菌株已针对与皮肤病相关的其他细菌进行了测试。乳酸杆菌目使用的对所有测试的潜在皮肤病原体都有活性。这些益生菌可以封装在聚合物膜之间——一个紧密，另一个对其产品具有渗透性，通过真空干燥保存，并在至少三个月储存后重新激活。重要的是，含有益生菌的重新激活垫在琼脂板上对所有测试的病原体都表现出抗菌活性。这表明含有益生菌的垫可以局部用于治疗皮肤病，而不需要常规抗生素治疗或作为辅助治疗。