Quercetin (QCT), existing in common dietary sources, is an abundant bioflavonoid with planar structure and exerts multiple pharmacological effects. Herein, four kinds of liposomes were prepared as model biomembranes, and then the partition coefficient, distribution in lipid membrane and influence of the QCT on the membrane properties were evaluated. The partition of QCT to lipid membranes was affected by both membrane phase state and the interference of QCT on membrane properties. The location of QCT in lipid membrane was related to the phase state of lipid membrane. In addition, influence of QCT on the compaction of the hydrocarbon tail in lipid membranes was dependent on the unsaturation degree of lipid molecules. Finally, about its antioxidant activity, from the results of 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay, it can be concluded that the interaction of QCT with lipid membrane greatly influences on QCT reductive activity in lipid membrane. Furthermore, mass spectrometry of DOPC molecule showed no lipid oxidation in the presence of QCT, indicating that in addition to the QCT ability toward radical scavenging, the ordering effect of QCT in unsaturated lipid membrane would be helpful to protect lipid membrane from oxidation by inhibiting radical diffusion (synergy effect). Based on lipid membrane analysis, our study made it clear that the effect of QCT on various lipid membrane and its relation with the antioxidant effect of QCT within lipid membrane. Therefore, our analytical method and findings would be also helpful for understanding the mechanism of other antioxidants effects on biomembrane.

槲皮素 (QCT) 存在于常见的膳食来源中，是一种丰富的具有平面结构的生物类黄酮，具有多种药理作用。在此，制备了四种脂质体作为模型生物膜，然后评估了分配系数、在脂质膜中的分布以及QCT对膜性质的影响。QCT 对脂质膜的分配受膜相状态和 QCT 对膜特性的干扰的影响。QCT在脂膜中的位置与脂膜的相态有关。此外，QCT 对脂质膜中碳氢化合物尾部压实的影响取决于脂质分子的不饱和度。最后，关于它的抗氧化活性，从 2,2-diphenyl-1-picrylhydrazyl 自由基清除试验的结果来看，可以得出结论，QCT与脂膜的相互作用对脂膜中QCT的还原活性有很大影响。此外，DOPC分子的质谱显示在QCT存在下没有脂质氧化，表明除了QCT清除自由基的能力外，QCT在不饱和脂质膜中的有序作用将有助于通过抑制自由基来保护脂质膜免受氧化。扩散（协同效应）。基于脂质膜分析，我们的研究明确了QCT对各种脂质膜的影响及其与脂质膜内QCT抗氧化作用的关系。因此，我们的分析方法和发现也将有助于了解其他抗氧化剂对生物膜的作用机制。