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A first approach to develop a quantitative screening method to identify resistance to bacterial leaf spot disease caused by Acidovorax anthurii in anthurium
European Journal of Plant Pathology ( IF 1.7 ) Pub Date : 2021-02-15 , DOI: 10.1007/s10658-021-02230-x
Annelle W. B. Holder , Winston Elibox , Pathmanathan Umaharan

Resistance of anthurium cultivars to infection by Acidovorax anthurii, the causal agent of bacterial leaf spot disease was investigated using seven inoculation methods, four inoculum concentrations and two different plant ages towards developing a screening method for identification of disease resistance. Injection of 1 mL of 1.5 × 109 CFU mL−1 three-quarter way down the intact petiole from the intact leaf lamina of a stage-4 leaf consistently and repeatedly elicited 100% infection in susceptible anthurium cultivars, and was the best inoculation method. All 9 measures of resistance using the optimized inoculation method were able to differentiate among 14 anthurium cultivars. Of the three most discriminating measures, proportion of infected leaves per plant showed the largest correlations with field resistance measures obtained through a farm survey and a field trial conducted in a sick plot, and was the best measure of resistance. The study provided evidence for two independent mechanisms of resistance, one at the level of entry and infection establishment and the other at the level of severity of infection. Only two anthurium cultivars showed resistance at both levels. In addition to a cultivar dependent mechanism of resistance, the study showed that there is a general mechanism of mature plant resistance. The study provides a quantitative screening method that can be used to breed for resistance to A. anthurii in anthurium.



中文翻译:

建立定量筛选方法以鉴定对由红掌中的Anthovorax anthurii引起的细菌性叶斑病的抗性的第一种方法

利用七种接种方法,四种接种物浓度和两种不同植物年龄,研究了红掌菌对细菌性叶斑病病原体的抗药性,以建立一种鉴定抗病性的筛选方法。注射1 mL的1.5×10 9  CFU mL -1从第4阶段叶片完整叶片的叶柄向下直至完整叶柄的四分之三处持续且反复地诱发易感红掌品种的100%感染,这是最好的接种方法。使用优化接种方法的所有9种抗性措施都能够在14个红掌品种之间进行区分。在这三种最有区别的措施中,每株植物被感染叶片的比例与通过农场调查和在病地进行的田间试验获得的田间抗性措施的相关性最大,并且是最佳的抗性度量。该研究为两种独立的耐药机制提供了证据,一种在进入和建立感染的水平上,另一种在感染的严重程度上。只有两个红掌品种在两个水平上均显示出抗性。除了抗性的依赖于品种的机制外,研究还显示了成熟植物抗性的一般机制。该研究提供了一种定量筛选方法,可用于繁殖抗性。红掌中的anthurii

更新日期:2021-04-15
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