Aurantio-obtusin (AO) is a major anthraquinone compound isolated from Cassiae Semen or Duhaldea nervosa, which possesses diverse pharmacological effects. Previous studies have shown that it has a good effect on lowering blood lipids and treating various diseases. A few studies have also reported about its metabolites. A rapid and reliable method using ultra-high-performance liquid chromatography-Q-Exactive Orbitrap mass spectrometry and multiple data-processing technologies was established to investigate the metabolites of AO in the plasma and various tissues of rats, including the heart, liver, spleen, lung, kidneys, and brain. Finally, a total of 36 metabolites were identified in the plasma of rats, which could be very beneficial for understanding the effective form of AO metabolites leading to new drug discovery. The result demonstrated that this strategy, especially parallel reaction monitoring, has shown a wide range of applications in the identification of metabolites.

中文翻译：

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超高效液相色谱-Q-活性Orbitrap质谱联用平行反应监测技术鉴定大鼠中奥兰托布汀的代谢产物

Aurantio-obtusin（AO）是一种主要的蒽醌化合物，从决明子或神经假体中分离出来。具有多种药理作用。先前的研究表明，它对降低血脂和治疗各种疾病有很好的效果。一些研究也报道了其代谢产物。建立了使用超高效液相色谱-Q-Exactive Orbitrap质谱和多种数据处理技术的快速可靠的方法，以研究大鼠血浆和心脏，肝脏，脾脏等组织中AO的代谢产物，肺，肾脏和大脑。最后，在大鼠血浆中总共鉴定出36种代谢物，这对于理解导致新药发现的AO代谢物的有效形式可能非常有益。结果表明，该策略，尤其是并行反应监控，