Recombinant inbred lines (RILs) developed from a cross between rust resistant (FLIP-2004-7L) and susceptible (L-9–12) genotypes were phenotyped against lentil rust at two hot-spot locations for two consecutive years (2017–2018 and 2018–2019) and analysed genetically for molecular map construction. Based on the mean score of both the locations, the RILs were classified into resistant and susceptible classes. The frequency distribution of disease severity in the RILs did not show a continuous variation, which strongly indicate the role of a major gene controlling the rust resistance. Resistant and susceptible bulks were constituted based on phenotypic data of RILs. Of 389 SSR markers, eight were found polymorphic between bulks. Genotyping of RILs with these eight markers revealed two markers, namely LcSSR440 and LcSSR606, showing co-segregation with rust resistance, which flanked the gene at 8.3 and 8.1cM, respectively. The applicability of these markers for marker-assisted breeding was further evaluated on a set of rust resistant and susceptible genotypes of lentil.

中文翻译：

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识别和验证与扁豆（Lens culinaris）抗锈病相关的 SSR 标记

由抗锈病 (FLIP-2004-7L) 和易感 (L-9-12) 基因型杂交产生的重组自交系 (RIL) 在两个热点地区连续两年（2017-2018 年和2018-2019）并进行基因分析以构建分子图谱。根据两个位置的平均得分，将 RIL 分为抗性和易感类。RILs中疾病严重程度的频率分布没有显示出连续变化，这强烈表明控制锈病抗性的主要基因的作用。基于 RILs 的表型数据构成抗性和易感性大块。在 389 个 SSR 标记中，发现了 8 个在大块之间具有多态性。用这八个标记对 RIL 进行基因分型揭示了两个标记，即 LcSSR440 和 LcSSR606，显示出与锈病抗性的共分离，分别在 8.3 和 8.1cM 的基因侧翼。在一组抗锈病和易感小扁豆基因型上进一步评估了这些标记在标记辅助育种中的适用性。