The extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) can be used as carriers for therapeutic molecules and drugs to target disordered tissues. This aimed to compare the protocols used for isolation of MSC-derived EVs by comparing EV collection conditions and three commercial purification kits. We also determined appropriate fluorescent dyes for labeling EVs. MSC-derived EVs were efficiently secreted during cell growth and highly purified by the phosphatidyl serine-based affinity kit. Although the EV membrane was more efficiently labeled with the fluorescent dye PKH67 compared to other probes, the efficiency was not enough to accurately analyze the endothelial cellular uptake of EVs. Results verified the easy protocol for isolating and fluorescently labeling EVs with commercial reagents and kits, but meanwhile, further modification of the protocol is required in order to scale up the amount of EVs derived from MSCs using fluorescent probes.

来自间充质干细胞 (MSCs) 的细胞外囊泡 (EVs) 可用作治疗分子和药物的载体，以靶向紊乱的组织。这旨在通过比较 EV 收集条件和三个商业纯化套件来比较用于分离 MSC 衍生 EV 的协议。我们还确定了用于标记 EV 的合适荧光染料。MSC 衍生的 EV 在细胞生长过程中有效分泌，并通过基于磷脂酰丝氨酸的亲和试剂盒高度纯化。尽管与其他探针相比，EV 膜被荧光染料 PKH67 标记的效率更高，但效率不足以准确分析 EV 的内皮细胞摄取。结果验证了使用商业试剂和试剂盒分离和荧光标记 EV 的简单方案，但同时，