Cell cultures have provided an ideal habitat for a wide variety of Mycoplasma and Acholeplasma species since the earliest days of in-vitro culture. The possibility of contamination with Spiroplasma species was addressed by Regulatory Authorities due to the increased commercial use of insect cells, recognising that Spiroplasmas have been isolated from many types of arthropod and also that insect cell cultures support Spiroplasma growth as they have been used for cultivation of fastidious species.

In this study we re-examined two cell culture samples previously confirmed as contaminated with mollicutes by cultural methods. One isolate had undergone sequencing which had placed it in the S. citri phylogenetic group, whilst the other had not been identified. Using modern sequencing methods we were able to further identify both isolates to species level.

自体外培养最早的日子以来，细胞培养已为各种支原体和无胆原体物种提供了理想的栖息地。由于昆虫细胞的商业用途增加，监管当局解决了螺旋原体物种污染的可能性，认识到螺旋原体已从许多类型的节肢动物中分离出来，并且昆虫细胞培养物支持螺旋原体的生长，因为它们已被用于培养挑剔的物种。

在这项研究中，我们重新检查了先前通过培养方法确认被软组织污染的两个细胞培养样品。一个分离株经过测序，将其置于S. citri系统发育组中，而另一个尚未鉴定。使用现代测序方法，我们能够在物种水平上进一步鉴定这两种分离株。