In this study, a simple method for the detection of a highly sensitive biomarker, based on nanostructure and paper-based to Surface-Enhanced Raman Spectroscopy (SERS) active substrates of varying grades per square metre (GSM), is introduced. To amplify the optical signal, gold nanostructures were employed as a carrier for signalling a specific antibody against insulin growth factor binding protein-3 (IGFBP-3). The horseradish peroxidase (HRP)-conjugated gold nanostructure catalytically oxidized the substrate, and HRP also strengthened the optical signals, representing the IGFBP-3 targeting quantity. Spherical gold nanoparticles GNP (40 nm) and gold nanostar GNS (40 nm) materials were altered in this study. In addition, in SERS evaluation and optimization, the paper-based SERS active substrates are not complicated to manufacture, are user-friendly, require small amounts of sample, and can use affordable solid SERS substrates. It is possible to efficiently use very active SERS substances, with enhancement factors (EF) of 55 × 10⁵, an LOD of 1.6 nM/mL, and GNS with 135 GSM printing paper. To the best of our knowledge, this is the first study to determine the concentration of IGFBP-3 using the SERS method.

在这项研究中，介绍了一种检测高灵敏度生物标志物的简单方法，该方法基于纳米结构和纸级至每平方米不同级别（GSM）的表面增强拉曼光谱（SERS）活性基质。为了放大光信号，采用金纳米结构作为载体，以信号传递针对胰岛素生长因子结合蛋白3（IGFBP-3）的特异性抗体。辣根过氧化物酶（HRP）共轭的金纳米结构催化氧化底物，并且HRP还增强了光信号，代表了IGFBP-3靶向量。在这项研究中改变了球形金纳米颗粒GNP（40 nm）和金纳米星GNS（40 nm）材料。此外，在SERS评估和优化过程中，纸质SERS活性基材的制造并不复杂，操作简便，需要少量样品，并且可以使用负担得起的固态SERS基材。可以有效地使用具有55×10的增强因子（EF）的非常活跃的SERS物质^{如图5所示}，LOD为1.6 nM / mL，并带有135 GSM打印纸的GNS。据我们所知，这是第一个使用SERS方法确定IGFBP-3浓度的研究。