Antipsychotics, including amisulpride (AMI), quetiapine (QUE), aripiprazole (ARI), and olanzapine (OLA), are used to treat mental illnesses associated with psychotic symptoms. The effect of these drugs on c-Fos expression in vasopressinergic (AVP) and oxytocinergic (OXY) neurons was studied in the hypothalamic paraventricular nucleus (PVN) of rats. The presence of c-Fos in AVP and OXY perikarya was investigated in seven PVN cells segregations: the anterior (Ant), dorsal cup (Dc), wing-shaped (Wi), periventricular zone (Pe), circle-shaped core (Co) and shell of core (Sh), and the posterior (pPVN) after an acute treatment with AMI-20 mg/kg, QUE-15 mg/kg, ARI-10 mg/kg, and OLA-5 mg/kg/bw in rats. Ninety min after treatments, the animals were sacrificed by transcardial perfusion with fixative and the PVN area sliced into 35 μm thick coronal sections for immunohistochemistry. The c-Fos was processed by avidin-biotin-peroxidase complex intensified with nickel-enhanced 3,3′-diaminobenzidine tetrahydrochloride. Visualization of AVP- and OXY-synthesizing neurons was achieved by a fluorescent marker Alexa Flour 568. The c-Fos-AVP and c-Fos-OXY colocalizations were evaluated from c-Fos stained sections merged with AVP or OXY ones. AMI, QUE, ARI, and OLA, single administration distinctly increased the c-Fos expression in each of the PVN cells segregations. QUE induced the highest magnitude of activation of AVP and OXY neurons, while OLA and AMI had only moderate effects. Incontestable variabilities detected in c-Fos expression in PVN AVP and OXY neurons extend the knowledge of selected antipsychotics extra-striatal actions and may also be helpful in a presumption of their possible functional impact.

抗精神病药，包括氨磺必利 (AMI)、喹硫平 (QUE)、阿立哌唑 (ARI) 和奥氮平 (OLA)，用于治疗与精神病症状相关的精神疾病。在大鼠的下丘脑室旁核 (PVN) 中研究了这些药物对血管加压素 (AVP) 和催产素 (OXY) 神经元中 c-Fos 表达的影响。在七个 PVN 细胞分离中研究了 AVP 和 OXY perikarya 中 c-Fos 的存在：前部 (Ant)、背杯 (Dc)、翼形 (Wi)、脑室周围区 (Pe)、圆形核心 (Co ) 和核壳 (Sh)，以及用 AMI-20 mg/kg、QUE-15 mg/kg、ARI-10 mg/kg 和 OLA-5 mg/kg/bw 急性治疗后的后壳 (pPVN)在大鼠中。治疗后九十分钟，用固定剂经心脏灌注处死动物，将 PVN 区域切成 35 μm 厚的冠状切片用于免疫组织化学。c-Fos 由抗生物素蛋白-生物素-过氧化物酶复合物处理，并用镍增强的 3,3'-二氨基联苯胺四盐酸盐强化。AVP 和 OXY 合成神经元的可视化是通过荧光标记 Alexa Flour 568 实现的。 c-Fos-AVP 和 c-Fos-OXY 共定位是从与 AVP 或 OXY 合并的 c-Fos 染色切片中评估的。AMI、QUE、ARI 和 OLA，单次给药明显增加了每个 PVN 细胞分离中的 c-Fos 表达。QUE 诱导了 AVP 和 OXY 神经元的最高激活幅度，而 OLA 和 AMI 仅具有中等影响。