Aspergillus neoniger NCIM 1400 whose cell-free fraction was earlier established for transglycosylation activity conferred by α-glucosidase gene (agdA), was subjected to sequence analysis. Preliminary results revealed certain dynamics in the intron splicing mechanism, and to ascertain these molecular events, a detailed study was carried. The electrophoresis results from the cDNA portion (B-fragment) of agdA showed multiple bands, indicating the amplification of one or more fragments. The sequence results of cDNA cloned vector revealed the retention type of alternative splicing in the agdA. The splicing mechanism of agdA in NCIM 1400 was compared to different A. niger strains, which harbours agdA orthologues, using PCR. It was observed that effective intron splicing leads to higher α-glucosidase activity from these selected Aspergillus spp. To explore the dynamics of intron retention in A. neoniger NCIM 1400, time-course analysis of intron retention, enzyme activity, and sugar consumption were carried over a period of 168 h of fungal growth. RT-qPCR results revealed that introns retention was not detected during the initial growth phase when the maltose and its hydrolysed product, glucose were consumed. Here we demonstrate that exhaustion of maltose causes increase in retention of introns in the mRNA transcripts of agdA gene, and this could be the possible mode of regulating this gene.

对新黑曲霉 NCIM 1400进行序列分析，其无细胞部分是较早建立的用于由 α-葡萄糖苷酶基因 ( agd A)赋予的转糖基化活性。初步结果揭示了内含子剪接机制的某些动态，为了确定这些分子事件，进行了详细的研究。agd A的 cDNA 部分（B 片段）的电泳结果显示多条带，表明一个或多个片段的扩增。的cDNA克隆载体的序列结果表明选择性剪接在保持型AGD A.的剪接机制AGD甲在NCIM 1400进行比较不同的黑曲霉菌株，其港口agd A 直向同源物，使用 PCR。观察到有效的内含子剪接导致来自这些选定曲霉属的更高的α-葡萄糖苷酶活性。为了探索A.neoniger NCIM 1400中内含子保留的动力学，在真菌生长的 168 小时内进行了内含子保留、酶活性和糖消耗的时程分析。RT-qPCR 结果显示，当麦芽糖及其水解产物葡萄糖被消耗时，在初始生长期未检测到内含子保留。在这里，我们证明麦芽糖耗尽导致agd A 基因mRNA 转录物中内含子的保留增加，这可能是调节该基因的可能模式。