Abstract

An innovative nano-base polymer that scavenges radicals and reactive oxygen species exhibits potential antibacterial properties, which are crucial in the biomedical field, particularly in reducing nosocomial infections. However, the safety of this nano-based polymer, which has direct contact with the human system, has not been fully understood. The present study investigated the cytocompatibility and hemocompatibility responses of linear low-density polyethylene polymer (LLDPE) embedded with difference ratios of heterogeneous TiO₂/ZnO nanocomposites. Exposure of the blood and fibroblast cells to LLDPE/100Z and LLDPE/25T75Z/10% nanocomposite films for 48 and 72 h decreased their viability by less than 40%, compared with LLDPE, LLDPE/100T and LLDPE/25T75Z/5% nanocomposite films. It also presented possible cellular damage and cytotoxicity, which was supported by the findings from the significant release of extracellular lactate dehydrogenase profiles and cell survival assay Further observation using an electron microscope revealed that LLDPE films with heterogeneous 25T75Z/5% promoted cell adhesion. Moreover, no hemolysis was detected in all ratios of heterogeneous TiO₂/ZnO nanocomposite in LLDPE film as it was less than 0.2%, suggesting that these materials were hemocompatible. This study on LLDPE film with heterogeneous TiO₂/ZnO nanocomposites demonstrated favorable biocompatible properties that were significant for advanced biomedical polymer application in a hospital setting.

摘要

一种清除自由基和活性氧的创新纳米基聚合物显示出潜在的抗菌特性，这在生物医学领域至关重要，尤其是在减少医院感染方面。然而，这种与人体系统直接接触的纳米聚合物的安全性尚未完全了解。本研究调查了嵌入不同比例的异质 TiO ₂的线性低密度聚乙烯聚合物 (LLDPE) 的细胞相容性和血液相容性反应。/ZnO 纳米复合材料。与 LLDPE、LLDPE/100T 和 LLDPE/25T75Z/5% 纳米复合薄膜相比，血液和成纤维细胞暴露于 LLDPE/100Z 和 LLDPE/25T75Z/10% 纳米复合薄膜 48 和 72 小时后，其活力降低了不到 40% . 它还提出了可能的细胞损伤和细胞毒性，这得到了细胞外乳酸脱氢酶谱的显着释放和细胞存活测定的结果的支持。使用电子显微镜的进一步观察表明，具有异质 25T75Z/5% 的 LLDPE 膜促进了细胞粘附。此外，LLDPE 薄膜中所有比例的异质 TiO ₂ /ZnO 纳米复合材料均未检测到溶血，因为它小于 0.2%，表明这些材料具有血液相容性。含异质TiO的LLDPE薄膜的研究₂ /ZnO 纳米复合材料表现出良好的生物相容性，这对于先进的生物医学聚合物在医院环境中的应用具有重要意义。