Graphene oxide (GO)-based hydrogel beads were synthesized by physical crosslinking of GO with carboxymethyl cellulose (CMC) to produce a pH-sensitive drug carrier with selective drug-release properties. Four different GO/CMC composite hydrogel beads (GCB) were prepared with GO oxidized at 30°C and 5q0°C with varying GO loading 1 or 5 mg/ml. Physicochemical properties of the synthesized GO and its composite beads were provided in this work. A mild oxidation temperature of 50°C produce GO-50 with satisfactory oxidation degree as shown by intensity ratio of D and G bands (I_D/I_G) and carbon to oxygen (C/O) ratios of 0.991 and 1.94, respectively. The highest loading capacity of doxorubicin (DOX) was 4.2494 mg/g for GCB synthesized from GO-50 with dispersion concentration of 5 mg/ml (GCB-50.5), corresponding to its abundant oxygen-containing functional groups. The release profile of DOX also confirmed its strong pH-sensitive behavior. The in vitro cytotoxicity tests on 7F2 cells by MTT assay revealed that GCBs have a higher percentage of viability than that of their GO precursors due to the incorporation of CMC into the beads. The prepared hydrogel beads thus can potentially be used as an effective and viable DOX carrier.

中文翻译：

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氧化石墨烯-羧甲基纤维素水凝胶珠用于阿霉素的吸收和释放研究

通过 GO 与羧甲基纤维素 (CMC) 的物理交联合成了基于氧化石墨烯 (GO) 的水凝胶珠，以产生具有选择性药物释放特性的 pH 敏感药物载体。四种不同的 GO/CMC 复合水凝胶珠粒 (GCB) 用 GO 在 30°C 和 5q0°C 下氧化，GO 负载量为 1 或 5 mg/ml。在这项工作中提供了合成的 GO 及其复合珠的物理化学性质。50°C 的温和氧化温度产生具有令人满意的氧化度的 GO-50，如 D 和 G 带的强度比（I _D /I _G) 和碳氧 (C/O) 比分别为 0.991 和 1.94。对于由GO-50合成的GCB（GCB-50.5），多柔比星（DOX）的最高负载量为4.2494 mg / g，分散浓度为5 mg / ml（GCB-50.5），对应于其丰富的含氧官能团。DOX 的释放曲线也证实了其强烈的 pH 敏感行为。通过 MTT 测定对 7F2 细胞进行的体外细胞毒性测试表明，由于 CMC 掺入珠中，GCB 具有比其 GO 前体更高的活力百分比。因此，制备的水凝胶珠有可能用作有效且可行的 DOX 载体。