Identification of peripheral oxytocin-expressing cells using systemically applied cell-type specific adeno-associated viral vector,Journal of Neuroendocrinology

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Identification of peripheral oxytocin-expressing cells using systemically applied cell-type specific adeno-associated viral vector
Journal of Neuroendocrinology ( IF 3.3 ) Pub Date : 2021-04-14 , DOI: 10.1111/jne.12970
Luis Paiva ₁ , Maja Lozic ₁ , Andrew Allchorne ₁ , Valery Grinevich ₂ , Mike Ludwig _{1,

3}

Affiliation

Oxytocin is primarily synthesised in the brain and is widely known for its role in lactation and parturition after being released into the blood from the posterior pituitary gland. Nevertheless, peripheral tissues have also been reported to express oxytocin. Using systemic injection of a recombinant adeno-associated virus vector, we investigated the expression of the green fluorescent protein Venus under the control of the oxytocin promoter in the gastrointestinal tract, pancreas and testes of adult rats. Here, we confirm that the vector infects oxytocin neurones of the enteric nervous system in ganglia of the myenteric and submucosal plexuses. Venus was detected in 25%-60% of the ganglia in the myenteric and submucosal plexuses identified by co-staining with the neuronal marker PGP9.5. Oxytocin expression was also detected in the islets of Langerhans in the pancreas and the Leydig cells of the testes. Our data illustrate that peripheral administration of the viral vector represents a powerful method for selectively labelling oxytocin-producing cells outside the brain.

中文翻译：

使用全身应用的细胞类型特异性腺相关病毒载体鉴定外周催产素表达细胞

催产素主要在大脑中合成，并因其从垂体后叶释放到血液中后在哺乳和分娩中的作用而广为人知。然而，据报道外周组织也表达催产素。通过全身注射重组腺相关病毒载体，我们研究了成年大鼠胃肠道、胰腺和睾丸中在催产素启动子控制下绿色荧光蛋白 Venus 的表达。在这里，我们确认该载体感染肠肌和黏膜下丛神经节中肠神经系统的催产素神经元。通过与神经元标记 PGP9.5 共染色，在肌间和黏膜下丛中 25%-60% 的神经节中检测到金星。在胰腺朗格汉斯小岛和睾丸间质细胞中也检测到催产素表达。我们的数据表明，病毒载体的外周给药代表了一种强有力的方法，用于选择性地标记脑外产生催产素的细胞。

更新日期：2021-05-25

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