Thioredoxins (Trxs) function within the antioxidant network through modulation of one or more redox reactions involved in oxidative-stress signaling. Given their function in regulating cellular redox, Trx proteins also fulfill key roles in plant immune signaling. Here, TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment. Overexpression of TaTrxh1 in tobacco (Nicotiana benthamiana) induced programmed cell death. Silencing of TaTrxh1 in wheat enhanced susceptibility to P. striiformis f. sp. tritici in different aspects, including reactive oxygen species accumulation and pathogen-responsive or -related gene expression. Herein, we observed that the cellular concentration of SA was significantly reduced in TaTrxh1-silenced plants, indicating that TaTrxh1 possibly regulates wheat resistance to stripe rust through a SA-associated defense signaling pathway. Using a yeast two-hybrid screen to identify TaTrxh1-interacting partners, we further show that interaction with TaCP1 (a RD19-like cysteine protease) and subsequent silencing of TaCP1 reduced wheat resistance to P. striiformis f. sp. tritici. In total, the data presented herein demonstrate that TaTrxh1 enhances wheat resistance against P. striiformis f. sp. tritici via SA-dependent resistance signaling and that TaTrxh1 interaction with TaCP1 is required for wheat resistance to stripe rust.

Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.

硫氧还蛋白（Trxs）通过调节参与氧化应激信号的一种或多种氧化还原反应而在抗氧化剂网络中起作用。鉴于其在调节细胞氧化还原中的功能，Trx蛋白在植物免疫信号传导中也起着关键作用。在这里，TaTrxh1，编码Trx家族的h亚型成员，被鉴定并克隆到小麦（Triticum aestivum）中，小麦被小麦条锈菌（Puccinia striiformis f ）快速诱导。sp。小麦的入侵和水杨酸（SA）处理。TaTrxh1在烟草（Nicotiana benthamiana）中的过表达诱导程序性细胞死亡。TaTrxh1的沉默小麦中小麦对条纹霉菌的敏感性增强。sp。小麦的不同方面，包括活性氧的积累和病原体反应性或相关基因的表达。在本文中，我们观察到在沉默了TaTrxh1的植物中SA的细胞浓度显着降低，这表明TaTrxh1可能通过与SA相关的防御信号传导途径调节小麦对条锈病的抗性。使用酵母双杂交筛选，以鉴定TaTrxh1相互作用伙伴，我们进一步显示与该TACP1相互作用（一个RD19样半胱氨酸蛋白酶）和随后的沉默TACP1减少到小麦电阻条锈菌F。sp。小麦。总体而言，本文提供的数据证明TaTrxh1增强了小麦对条锈菌f。的抗性。sp。小麦通过抗SA信号传递抗性小麦，并且TaTrxh1与TaCP1的相互作用对于小麦抗条锈病是必需的。

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