Enhancement of angiogenin inhibition by polyphenol-capped gold nanoparticles,Biopolymers

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Enhancement of angiogenin inhibition by polyphenol-capped gold nanoparticles
Biopolymers ( IF 3.2 ) Pub Date : 2021-04-14 , DOI: 10.1002/bip.23429
Atashi Panda ₁ , Siddhant Karhadkar ₁ , Bidisha Acharya ₂ , Anwesha Banerjee ₁ , Soumya De ₂ , Swagata Dasgupta ₁

Affiliation

Angiogenin (Ang), is a ribonucleolytic protein that is associated with angiogenesis, the formation of blood vessels. The involvement of Ang in vascularisation makes it a potential target for the identification of compounds that have the potential to inhibit the process. The compounds may be assessed for their ability to inhibit the ribonucleolytic activity of the protein and subsequently blood vessel formation, a crucial requirement for tumor formation. We report an inhibition of the ribonucleolytic activity of Ang with the gallate containing green tea polyphenols, ECG and EGCG that exhibits an increased efficacy upon forming polyphenol-capped gold nanoparticles (ECG-AuNPs and EGCG-AuNPs). The extent of inhibition was confirmed using an agarose gel-based assay followed by fluorescence titration studies that indicated a hundred fold stronger binding of polyphenol-capped gold nanoparticles (GTP-AuNPs) compared to the bare polyphenols. Interestingly, we found a change in the mode of inhibition from a noncompetitive type to a competitive mode of inhibition in case of the GTP-AuNPs, which is in agreement with the 'n' values obtained from the fluorescence quenching studies. The effect on angiogenesis has also been assessed by the chorioallantoic membrane (CAM) assay. We find an increase in the inhibition potency of GTP-AuNPs that could find applications in the development of anti-angiogenic compounds.

中文翻译：

多酚封端的金纳米粒子增强血管生成素抑制作用

血管生成素 (Ang) 是一种核糖核酸分解蛋白，与血管生成、血管形成有关。Ang 参与血管形成使其成为识别具有抑制该过程潜力的化合物的潜在目标。可以评估化合物抑制蛋白质的核糖核酸分解活性和随后血管形成的能力，这是肿瘤形成的关键要求。我们报告了含有绿茶多酚、ECG 和 EGCG 的没食子酸酯对 Ang 的核糖核酸分解活性的抑制，其在形成多酚封端的金纳米粒子（ECG-AuNPs 和 EGCG-AuNPs）时表现出更高的功效。抑制程度通过基于琼脂糖凝胶的测定和荧光滴定研究得到证实，该研究表明与裸多酚相比，多酚封端的金纳米粒子 (GTP-AuNPs) 的结合强度要强 100 倍。有趣的是，我们发现在 GTP-AuNPs 的情况下，抑制模式从非竞争性类型转变为竞争性抑制模式，这与从荧光猝灭研究中获得的n ' 值。还通过绒毛膜尿囊膜 (CAM) 测定评估了对血管生成的影响。我们发现 GTP-AuNPs 的抑制效力增加，可以在抗血管生成化合物的开发中找到应用。

更新日期：2021-04-14

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