Abstract

An in-fiber optofluidic chemiluminescence (CL) device is reported based on a low-cost microstructured polymer optical fiber (mPOF). The mPOF is composed of a central hole for liquid delivery and a suspended core for optical coupling. Due to the lower processing temperature of mPOF, the difficulty of constructing microfluidic channels is greatly reduced. In addition, the large core diameter of mPOF is more conducive to optical coupling. The microholes are opened on the surface of the mPOF by partial melting to complete the construction of the microfluidic channel. Simultaneously, the response of the CL reaction is detected on-line by the optoelectronic device. Minocycline (MC) was employed as the analyte for on-line optofluidic CL detection. The results show that the device detects aqueous minocycline from 0.0001 to 0.1 g/L with a linear response between 0.001 and 0.01 g/L. The limit of detection is 0.0001 g/L. Moreover, the ability of the device to detect minocycline in blood serum is investigated. The relative deviation from the linear calibration value is within 3.2%. Furthermore, the low-cost in-fiber optofluidic CL device may be integrated with other devices and offers potential in drug safety and medical detection.

摘要

据报道，基于低成本微结构聚合物光纤 (mPOF) 的光纤内光流体化学发光 (CL) 装置。mPOF 由一个用于液体输送的中心孔和一个用于光耦合的悬浮芯组成。由于mPOF的加工温度较低，微流控通道的构建难度大大降低。此外，mPOF的大芯径更有利于光耦合。通过部分熔化在 mPOF 表面打开微孔以完成微流体通道的构建。同时，光电子装置在线检测CL反应的响应。米诺环素 (MC) 被用作在线光流控 CL 检测的分析物。结果表明，该装置可从 0.0001 到 0 检测水性米诺环素。1 g/L，线性响应介于 0.001 和 0.01 g/L 之间。检测限为 0.0001 g/L。此外，研究了该装置检测血清中米诺环素的能力。与线性校准值的相对偏差在 3.2% 以内。此外，低成本的光纤内光流体 CL 设备可以与其他设备集成，并在药物安全和医学检测方面提供潜力。