ABSTRACT

Catharanthus roseus (Madagascar periwinkle) plants exhibiting typical begomovirus symptoms, i.e., leaf yellowing and curling were found in Lakshmangarh, Rajasthan, India. Symptomatic and healthy leaf samples were collected and subjected to DNA extraction and polymerase chain reaction, using universal primers conserved for the coat protein region of begomoviruses. The full length of viral DNA was amplified by rolling circular amplification (RCA) followed by cloning and sequencing. We identified the viral DNA sequence as papaya leaf curl virus (PaLCuV: KX353619) and papaya leaf curl betasatellite (PaLCuB: KX353621). Analysis of complete viral DNA component revealed the highest similarity (93%) with Papaya leaf curl virus, while sequence data of betasatellite showed maximum (93%) identity with Papaya leaf curl betasatellite. To our knowledge, this is the first account of these two types of geminivirus DNA molecules harbouring together in C. roseus plants.

摘要

在印度拉贾斯坦邦的 Lakshmangarh 发现了表现出典型 begomovirus 症状，即叶片变黄和卷曲的长春花（马达加斯加长春花）植物。收集有症状和健康的叶子样本，并使用为 begomoviruses 外壳蛋白区域保守的通用引物进行 DNA 提取和聚合酶链反应。病毒 DNA 的全长通过滚动循环扩增 (RCA) 进行扩增，然后进行克隆和测序。我们将病毒 DNA 序列鉴定为木瓜卷叶病毒（PaLCuV：KX353619）和木瓜卷叶β卫星（PaLCuB：KX353621）。完整的病毒 DNA 成分分析显示与木瓜卷叶病毒的相似性最高 (93%) ，而 β 卫星的序列数据显示与木瓜叶卷曲β卫星。据我们所知，这是对这两种类型的双生病毒 DNA 分子共同存在于玫瑰花植物中的首次描述。