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A Standard Protocol for the Production and Bioevaluation of Ethical In Vivo Models of HPV-Negative Head and Neck Squamous Cell Carcinoma
ACS Pharmacology & Translational Science ( IF 4.9 ) Pub Date : 2021-04-14 , DOI: 10.1021/acsptsci.1c00083 Patrizia Sarogni 1 , Ana Katrina Mapanao 1, 2 , Sabrina Marchetti 3 , Claudia Kusmic 3 , Valerio Voliani 1
ACS Pharmacology & Translational Science ( IF 4.9 ) Pub Date : 2021-04-14 , DOI: 10.1021/acsptsci.1c00083 Patrizia Sarogni 1 , Ana Katrina Mapanao 1, 2 , Sabrina Marchetti 3 , Claudia Kusmic 3 , Valerio Voliani 1
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Preclinical cancer research increasingly demands sophisticated models for the development and translation of efficient and safe cancer treatments to clinical practice. In this regard, tumor-grafted chorioallantoic membrane (CAM) models are biological platforms that account for the dynamic roles of the tumor microenvironment and cancer physiopathology, allowing straightforward investigations in agreement to the 3Rs concept (the concept of reduction, refinement, and replacement of animal models). CAM models are the next advanced model for tumor biological explorations as well as for reliable assessment regarding initial efficacy, toxicity, and systemic biokinetics of conventional and emerging neoplasm treatment modalities. Here we report a standardized and optimized protocol for the production and biocharacterization of human papillomavirus (HPV)-negative head and neck chick chorioallantoic membrane models from a commercial cell line (SCC-25). Oral malignancies continue to have severe morbidity with less than 50% long-term survival despite the advancement in the available therapies. Thus, there is a persisting demand for new management approaches to establish more efficient strategies toward their treatment. Remarkably, the inclusion of CAM models in the preclinical research workflow is crucial to ethically foster both the basic and translational oncological research on oral malignancies as well as for the advancement of efficient cancer treatment approaches.
中文翻译：
HPV 阴性头颈鳞状细胞癌伦理体内模型的制作和生物评价标准方案
临床前癌症研究越来越需要复杂的模型来开发高效、安全的癌症治疗并将其转化为临床实践。在这方面，肿瘤移植绒毛尿囊膜 (CAM) 模型是解释肿瘤微环境和癌症病理生理学动态作用的生物平台，允许根据 3R 概念（减少、细化和替换动物模型）。CAM 模型是肿瘤生物学探索以及传统和新兴肿瘤治疗方式的初始疗效、毒性和系统生物动力学的可靠评估的下一个先进模型。在这里，我们报告了一种标准化和优化的方案，用于从商业细胞系（SCC-25）中生产人乳头瘤病毒（HPV）阴性头颈雏鸡绒毛尿囊膜模型并进行生物表征。尽管现有疗法取得了进步，但口腔恶性肿瘤的发病率仍然很高，长期生存率低于 50%。因此，持续需要新的管理方法来制定更有效的治疗策略。值得注意的是，将 CAM 模型纳入临床前研究工作流程对于在伦理上促进口腔恶性肿瘤的基础和转化肿瘤学研究以及推进有效的癌症治疗方法至关重要。
更新日期：2021-06-11
中文翻译：
HPV 阴性头颈鳞状细胞癌伦理体内模型的制作和生物评价标准方案
临床前癌症研究越来越需要复杂的模型来开发高效、安全的癌症治疗并将其转化为临床实践。在这方面，肿瘤移植绒毛尿囊膜 (CAM) 模型是解释肿瘤微环境和癌症病理生理学动态作用的生物平台，允许根据 3R 概念（减少、细化和替换动物模型）。CAM 模型是肿瘤生物学探索以及传统和新兴肿瘤治疗方式的初始疗效、毒性和系统生物动力学的可靠评估的下一个先进模型。在这里，我们报告了一种标准化和优化的方案，用于从商业细胞系（SCC-25）中生产人乳头瘤病毒（HPV）阴性头颈雏鸡绒毛尿囊膜模型并进行生物表征。尽管现有疗法取得了进步，但口腔恶性肿瘤的发病率仍然很高，长期生存率低于 50%。因此，持续需要新的管理方法来制定更有效的治疗策略。值得注意的是，将 CAM 模型纳入临床前研究工作流程对于在伦理上促进口腔恶性肿瘤的基础和转化肿瘤学研究以及推进有效的癌症治疗方法至关重要。
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