Abstract

DNA methylation could take part in the gene expression and acts an important role in muscle development. In this study, DNA methylation and expression in adipose and muscle tissues were examined at the same time to evaluate the extent of epigenetic modifications and gene expression on the differentially methylated region (DMR) in SERPINA3. Chain reaction of bisulfite sequencing polymerase (BSP) was used to compared difference among DNA methylation patterns. The result of quantitative real-time PCR (qPCR) analysis showed that there was an extensive expression of SERPINA3 gene in tissue and there was a significant difference existing in muscle and adipose between Jiaxian cattle and individual of other breeds with increasing hybridization (p < 0.05). The statistic analyses indicated that DNA methylation patterns had a significant influence to the level of mRNA in tissue of fat and muscle. This study may be an important reference for investigating development of muscle tissue in cattle, and may promote the process of cattle molecular breeding.

摘要

DNA甲基化可参与基因表达并在肌肉发育中起重要作用。在这项研究中，同时检查了脂肪和肌肉组织中的 DNA 甲基化和表达，以评估SERPINA3中差异甲基化区域 (DMR) 的表观遗传修饰和基因表达的程度。亚硫酸氢盐测序聚合酶 (BSP) 的链式反应用于比较 DNA 甲基化模式之间的差异。实时定量PCR（qPCR）分析结果表明，SERPINA3基因在组织中大量表达，随着杂交程度的增加，嘉县牛与其他品种个体的肌肉和脂肪存在显着差异（p < 0.05)。统计分析表明，DNA甲基化模式对脂肪和肌肉组织中的mRNA水平有显着影响。该研究可能为研究牛肌肉组织的发育提供重要参考，并可能促进牛分子育种的进程。