Objective: Brain-derived neurotrophic factor (BDNF) dysregulation is widely related with various psychiatric and neurological disorders, including schizophrenia, depression, Rett syndrome, and addiction, and the available evidence suggests that BDNF is also highly correlated with Parkinson’s and Alzheimer’s diseases. Methods: The BDNF target sequence was detected on a capture probe attached on aluminum microcomb electrodes on the silicon wafer surface. A capture-target-reporter sandwich-type assay was performed to enhance the detection of the BDNF target. Results: The limit of detection was noticed to be 100 aM. Input of a reporter sequence at concentrations #x3e;10 aM improved the detection of the target sequence by enhancing changes in the generated currents. Control experiments with noncomplementary and single- and triple-mismatches of target and reporter sequences did not elicit changes in current levels, indicating the selective detection of the BDNF gene sequence. Conclusion: The above detection strategy will be useful for the detection and quantification of BDNF, thereby aiding in the provision of suitable treatments for BDNF-related disorders.
Dev Neurosci

中文翻译：

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硅片上铝微梳电极用于检测脑源性神经营养因子中 Val66Met 多态性

目的：脑源性神经营养因子（BDNF）失调与精神分裂症、抑郁症、雷特综合征和成瘾等各种精神和神经系统疾病广泛相关，现有证据表明，脑源性神经营养因子（BDNF）与帕金森病和阿尔茨海默病也高度相关。方法： BDNF 目标序列在连接在硅晶片表面的铝微梳电极上的捕获探针上检测。进行了捕获-目标-报告分子夹心型测定以增强对 BDNF 目标的检测。结果：注意到检测限为上午 100 点。以 #x3e;10 aM 的浓度输入报告序列通过增强生成电流的变化来改进目标序列的检测。目标和报告序列的非互补和单一和三重错配的对照实验没有引起当前水平的变化，表明选择性检测了 BDNF 基因序列。结论：上述检测策略将有助于BDNF的检测和量化，从而有助于为BDNF相关疾病提供合适的治疗方法。
开发神经科学