A natural biodegradable polymer, polyhydroxyalkanoate (PHA), was adjuvanted with a vaccine seed to observe the biomaterial's ability in enhancing an immune response in rats. The adjuvant potential of PHA was tested using the whole-killed Pasteurella multocida B:2 (PMB2) vaccine in Sprague Dawley (SD) rats to detect changes in serum immunoglobulin G (IgG) and immunoglobulin M (IgM) responses. A common PHA, poly(3-hydroxybutyrate) [P(3HB)], from Bacillus megaterium UMTKB-1 was constructed into microparticles using the solvent evaporation method. Twelve SD rats were divided into four treatment groups: 1) non-treatment as negative control, 2) P(3HB) adjuvant, 3) PMB2 vaccine, and 4) adjuvanted-P(3HB)/PMB2 vaccine groups, which were intramuscularly vaccinated twice. Immunoglobulins IgG and IgM levels were used as markers of the immune response induced by the adjuvanted-P(3HB)/PMB2 vaccine and analysed over an eight-week study period. The group vaccinated specifically with adjuvanted-P(3HB)/PMB2 vaccine had higher concentrations of immunoglobulins compared to other treatment groups, hence demonstrating the potential of the adjuvant to enhance immune response. Findings showed a need to delay the delivery of the second booster dose to determine the appropriate regime for the adjuvanted-P(3HB)/PMB2 vaccine.

天然可生物降解聚合物聚羟基链烷酸酯 (PHA) 与疫苗种子相结合，以观察该生物材料增强大鼠免疫反应的能力。在 Sprague Dawley (SD) 大鼠中使用全灭的多杀巴斯德氏菌B:2 (PMB2) 疫苗测试 PHA 的佐剂潜力，以检测血清免疫球蛋白 G (IgG) 和免疫球蛋白 M (IgM) 反应的变化。一种常见的 PHA，聚（3-羟基丁酸）[P(3HB)]，来自巨大芽孢杆菌使用溶剂蒸发法将 UMTKB-1 构建成微粒。12只SD大鼠分为4个治疗组：1）未治疗作为阴性对照，2）P（3HB）佐剂，3）PMB2疫苗，4）佐剂-P（3HB）/PMB2疫苗组，肌肉注射两次。免疫球蛋白 IgG 和 IgM 水平被用作佐剂-P(3HB)/PMB2 疫苗诱导的免疫反应的标志物，并在八周的研究期内进行分析。与其他治疗组相比，专门接种佐剂 P(3HB)/PMB2 疫苗的组具有更高浓度的免疫球蛋白，因此证明了佐剂增强免疫反应的潜力。结果表明需要推迟第二次加强剂量的给药，以确定佐剂-P(3HB)/PMB2 疫苗的适当方案。