To promote the application of Trichoderma, many countries have collected Trichoderma resources. In the present study, nine isolates were isolated from a rhizosphere soil of Phellodendron amurense and were identified as three species: Trichoderma brevicompactum (one isolate), T. asperellum (two isolates), T. atroviride (six isolates). Dual culture experiments showed that T. asperellum T-Pa2 grew fast and produced the best inhibition rates against six tested pathogens (80.25–91.65%) via competition and mycoparasitism. Populus davidiana × P. alba var. pyramidalis Louche (PdPap poplar) was treated with T-Pa2, which increased the catalase activity, nitrate reductase activity, and content of osmosis molecules significantly (P < 0.05). Meanwhile, induction by T-Pa2 increased the resistance of PdPap poplar against Alternaria alternata via modulating the expression of salicylic acid, jasmonic acid, and auxin transduction pathway genes. The results will form the basis for the collection and application of biocontrol agents in forestry.

为了促进木霉的应用，许多国家已经收集了木霉资源。在本研究中，九个分离物从的根际土壤中分离黄檗和被确定为三种：木霉属短密（一个分离株），T.棘孢（两种分离物），T.深绿（6株）。双重培养实验表明，曲霉菌T-Pa2生长迅速，并通过竞争和支原体寄生虫对6种被测病原体（80.25–91.65％）产生了最佳的抑制率。杨（Populus davidiana）  × 阿尔法（P. alba var。）锥体用T-Pa2处理Louche（PdPap杨树），可显着提高过氧化氢酶活性，硝酸盐还原酶活性和渗透分子的含量（P  <0.05）。同时，T-Pa2诱导通过调节水杨酸，茉莉酸和植物生长素转导途径基因的表达，提高了PdPap杨树对链格孢的抵抗力。研究结果将为林业中生物防治剂的收集和应用奠定基础。