Abstract

Objective

The effect of Alstonia boonei fractions on glucose homeostasis was investigated via in vitro enzyme inhibition activity, ex vivo glucose uptake assay, and in vivo methods in diabetic rats.

Methodology

A. boonei fractions were subjected to in vitro α-glucosidase inhibitory assay and then ex vivo glucose uptake activity. The butanol fraction of the leaves (ABBF) was picked for the in vivo assay since it showed more activity in the initial tests conducted. ABBF was administrated via oral dosing to six-weeks old fructose-fed STZ-induced type 2 diabetic rats over a 5-week experimental period.

Results

ABBF treatment at a low dose of 150 mg/kg bw, significantly (p < .05) reduced blood glucose level, enhanced oral glucose tolerance ability, restored insulin secretion and hepatic glycogen synthesis as well as promoted islet regeneration than the high dose (300 mg/kg bw).

Conclusion

These results suggest that ABBF could be exploited as a therapeutic potential for treating T2D.

中文翻译：

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Alstonia boonei De Wild 的丁醇馏分。叶子改善糖尿病大鼠的氧化应激并调节关键的低血糖过程

摘要

客观的

通过糖尿病大鼠的体外酶抑制活性、离体葡萄糖摄取测定和体内方法，研究了Alstonia boonei组分对葡萄糖稳态的影响。

方法

对A. boonei 级分进行体外α-葡萄糖苷酶抑制测定，然后进行离体葡萄糖摄取活性。叶子的丁醇部分（ABBF）被挑选用于体内测定，因为它在最初进行的测试中显示出更多的活性。在为期 5 周的实验期间，通过口服给药的方式给六周大果糖喂养的 STZ 诱导的 2 型糖尿病大鼠施用 ABBF。

结果

ABBF低剂量150 mg/kg bw治疗，与高剂量（300 mg/kg bw）相比，显着（p  < .05）降低血糖水平，增强口服葡萄糖耐量能力，恢复胰岛素分泌和肝糖原合成，并促进胰岛再生。毫克/千克体重）。

结论

这些结果表明 ABBF 可以被用作治疗 T2D 的治疗潜力。