In this study, a fungal cellulase enzyme was applied to improve the selective separation of apatite from hematite during the reverse anionic flotation of a high-phosphorus iron ore. Bench-scale flotation tests were conducted on pure hematite and apatite minerals, their artificial mixture, and a high-phosphorus iron ore sample, using oleic acid as a collector. Flotation tests on single minerals showed that cellulase was a good depressant of hematite, but had no depressing effect on apatite at pH 10. The phosphorus content in the concentrate product of the flotation of artificially mixed pure minerals was reduced from 1.96% to 0.03%, with iron grade and recovery of 64.15% and 94.74%, respectively, using 4x10⁻⁵ mol/L oleate and 0.02% cellulase at pH 10. By applying the optimum separation conditions to the flotation of a natural high-phosphorus iron ore, the phosphorus content in the concentrate was reduced from 0.84% to 0.19% at an iron recovery of 67.85%. The mechanism of the cellulase depressing effect was investigated through Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) analyses. The results showed that cellulase enzyme was adsorbed on the surfaces of both minerals; however, the depressing effect was significant in case of hematite but not apatite. This might be attributed to the coverage of apatite surface with a close-packed layer of oleate molecules, which made the surface of apatite hydrophobic enough to counter the polarity induced upon cellulase adsorption.

在这项研究中，使用了一种真菌纤维素酶来改善高磷铁矿石的反向阴离子浮选过程中磷灰石与赤铁矿的选择性分离。使用油酸作为捕收剂，对纯赤铁矿和磷灰石矿物，它们的人工混合物和高磷铁矿石样品进行了基准规模的浮选试验。单一矿物的浮选试验表明，纤维素酶是赤铁矿的良好抑制剂，但在pH 10时对磷灰石没有抑制作用。人工混合的纯矿物浮选精矿产品中的磷含量从1.96％降低至0.03％，使用4x10 ^-5的铁品位和回收率分别为64.15％和94.74％摩尔/升油酸盐和0.02％的纤维素酶（pH值为10）。通过将最佳分离条件应用于浮选天然高磷铁矿石，铁的回收率达到67.85时，精矿中的磷含量从0.84％降低至0.19％。 ％。通过傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）分析研究了纤维素酶抑制作用的机理。结果表明，纤维素酶被两种矿物质的表面吸附。然而，对于赤铁矿而不是磷灰石，其抑制作用是显着的。这可能归因于油酸盐分子紧密堆积的层覆盖了磷灰石表面，这使磷灰石表面具有足够的疏水性以抵消纤维素酶吸附引起的极性。