The number of central and peripheral B cells and their responsiveness are decreased in aged mice. The diversity of mice central and peripheral B cell repertoires with increasing age has not been elucidated. In this study, we demonstrated that there were significant differences in the usage of some V, D, and J genes in the BCR H-CDR3 repertoire of bone marrow B cells, spleen B cells and spleen memory B cells in 3-, 12-, and 20-month-old mice. In the productive, pseudogene, and out-of-frame sequences, bone marrow B cells had significant differences in 5′J trimming with age; peripheral spleen B cells and memory B cells had significant differences in N1 insertion, N2 insertion, P5’D insertion, and 5’D trimming with age. The BCR H-CDR3 repertoire diversity of mice bone marrow B cells, spleen B cells and spleen memory B cells decreased with increasing age. The proportion of overlap in bone marrow and spleen B cells, but not spleen memory B cells, of mice at different ages was lower at 3 months than at 12 and 20 months. This study is the first to report the homogeneity and heterogeneity of the CDR3 repertoire of central and peripheral B cells change as mice age, to further investigation of the decline and response of B cell immunity in young/middle/old-aged mice.

中文翻译：

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在3、12和20个月大的小鼠的骨髓和脾脏中BCR H-CDR3库的异质性分析

在衰老小鼠中，中央和外周B细胞的数量及其反应性降低。尚未阐明随着年龄的增长小鼠中枢和外周B细胞组成的多样性。在这项研究中，我们证明了在3、12-和20个月大的小鼠。在生产性，假基因和框架外序列中，随着年龄的增长，骨髓B细胞在5'J修剪中具有显着差异。随着年龄的增长，外周脾B细胞和记忆B细胞在N1插入，N2插入，P5'D插入和5'D修剪方面具有显着差异。小鼠骨髓B细胞的BCR H-CDR3血库多样性，脾脏B细胞和脾脏记忆B细胞随着年龄的增长而减少。3个月时，不同年龄小鼠的骨髓和脾脏B细胞重叠而不是脾脏记忆B细胞重叠的比例低于12个月和20个月时。这项研究是第一个报告中枢和外周B细胞CDR3组成随着小鼠年龄而变化的同质性和异质性，以进一步研究年轻/中/老年小鼠中B细胞免疫力的下降和反应的方法。