Abstract—

The mechanisms of biocontrol action of the strains Pantoea brenneri AS3 and Bacillus ginsengihumi M2.11 were investigated. The ability of the strains to release ammonium and cyanide ions was established, as well as their cellulase and protease activity and ability to fix nitrogen and to mobilize soil phosphates and phytates. The strains were able to synthesize siderophores, with their maximum production observed 48 h of cultivation reaching 198 ± 8 μM in B. ginsengihumi strain M2.11 and 84 ± 7 μM in P. brenneri strain AS3. The bacteria produced a phytohormone, indoleacetic acid (IAA), with the yields of 34 ± 3 and and 29 ± 2 μg/mL for B. ginsengihumi M2.11 and P. brenneri AS3, respectively, after 24 h of growth. It was found that both strains had fungicidal activity against plant pathogens of the genus Fusarium the growth of different Fusarium species was inhibited by more than 90% in the presence of P. brenneri AS3 or B. ginsengihumi M2.11. It was concluded that the strains P. brenneri AS3 and B. ginsengihumi M2.11, which possess multiple characteristics useful to plants, may be used as objects for creating biofertilizers and plant growth stimulators.

中文翻译：

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Pantoea brenneri AS3和Bacillus ginsengihumi M2.11作为潜在的生物防治和植物生长促进剂

摘要—

研究了布氏泛菌AS3和人芽孢杆菌M2.11菌株的生物防治作用机理。确定了菌株释放铵离子和氰化物离子的能力，以及它们的纤维素酶和蛋白酶活性以及固定氮和动员土壤磷酸盐和植酸盐的能力。该菌株能够合成铁载体，观察到它们的最大产量在48小时的培养中在B.ginsengihumi菌株M2.11中达到198± 8μM，在P.brenneri菌株AS3中达到84± 7μM。该细菌产生了植物激素，吲哚乙酸（IAA），对人参芽孢杆菌M2.11和P的产率分别为34±3和29±2μg/ mL 。布伦内里生长24小时后，分别为AS3。发现这两种菌株对镰刀菌属的植物病原体均具有杀真菌活性，在存在布鲁氏假单胞菌AS3或人参B.ginsengihumi M2.11的情况下，不同镰刀菌物种的生长被抑制了90％以上。结论是，具有对植物有用的多种特征的菌株P.brenneri AS3和B.ginsengihumi M2.11可用作生产生物肥料和植物生长刺激物的对象。