The ability to maintain viable cultures of mature, primary cardiomyocytes is challenging. The lack of viable cardiomyocyte cultures severely limits in vitro biochemical assays, toxicology assays, drug screening assays, and other analyses. Here, we describe a novel three-dimensional (3D) embryonic scaffold, which supports the culture of postnatal day 7 murine cardiomyocytes within the embryonic heart for, at least, 28 days. We have observed that these cardiomyocytes display normal differentiation, protein expression, and function after extended culture. This novel culture system will allow for prolonged treatment of cardiomyocytes in a natural 3D orientation and has the potential for providing a superior tool for the screening of therapeutic compounds.

中文翻译：

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用于长期心肌细胞培养的生物衍生、三维、胚胎支架

维持成熟的原代心肌细胞的可行培养物的能力具有挑战性。缺乏可行的心肌细胞培养物严重限制了体外生化测定、毒理学测定、药物筛选测定和其他分析。在这里，我们描述了一种新的三维 (3D) 胚胎支架，它支持在胚胎心脏内培养出生后第 7 天的小鼠心肌细胞至少 28 天。我们观察到这些心肌细胞在延长培养后表现出正常的分化、蛋白质表达和功能。这种新型培养系统将允许在自然 3D 方向上对心肌细胞进行长期治疗，并有可能为筛选治疗化合物提供卓越的工具。