The present work evaluated the daily addition of probiotics (Bacillus subtilis and Bacillus licheniformis), during the rearing of Macrobrachium rosenbergii juveniles in a biofloc system for 40 days. Juveniles (0.12 ± 0.32 g) were stocked in 16 experimental units with bottom area of 0.20 m², 50 L of useful volume, and an equivalent density of 150 prawns m⁻². Four treatments were evaluated: Control (without addition of probiotics), TP1 (1.08 × 10⁵ CFU g⁻¹), TP2 (2.17 × 10⁵ CFU g⁻¹) and TP3 (3.25 × 10⁵ CFU g⁻¹). The treatments were randomly assigned to the experimental units with four replicates each. For quantitative evaluation of probiotic bacteria in the rearing water and in the hepatopancreas of prawns, microbiological analyses were carried out at days 0, 20 and 40. The results indicated a predominance of B. subtilis in the hepatopancreas and B. licheniformis in the water. The bacteria were differentiated by colony morphology and confirmed by Polymerase Chain Reaction (PCR) using species-specific primers. This study demonstrated that concentrations as low as 1.08 × 10⁵ CFU g⁻¹ (TP1) contribute to significantly higher survival (p = 0.014) of M. rosenbergii in Biofloc Technology System (BFT).

中文翻译：

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益生菌在生物絮团系统中养殖罗氏沼虾淡水虾（德曼，1879 年）

目前的工作评估了在生物絮凝系统中饲养罗氏沼虾幼鱼 40 天期间每天添加的益生菌（枯草芽孢杆菌和地衣芽孢杆菌）。将幼虾（0.12 ± 0.32 g）放养在 16 个实验单元中，底部面积为 0.20 m ²，有用体积为 50 L，当量密度为 150 对虾 m ^-2。评估了四种处理：对照（​​不添加益生菌）、TP1（1.08 × 10 ⁵  CFU g ^-1）、TP2（2.17 × 10 ⁵  CFU g ^-1）和TP3（3.25 × 10 ⁵  CFU g ^{-1 ）}）。处理随机分配到实验单元，每个单元重复四次。为了定量评价养殖水和对虾肝胰腺中的益生菌，在第 0、20 和 40 天进行了微生物分析。结果表明B占优势。 肝胰腺中的枯草杆菌和B . 地衣在水中。通过菌落形态对细菌进行区分，并使用物种特异性引物通过聚合酶链反应 (PCR) 进行确认。该研究表明，低至 1.08 × 10 ⁵  CFU g ^-1 (TP1) 的浓度有助于显着提高M 的存活率 ( p  = 0.014). 生物絮团技术系统 (BFT) 中的罗氏沼虾。