ABSTRACT

Transferring plants between different nutritional media is a common technique in plant nutrition research. Solidified sterile media are often used for plant culture and subsequent transfer experiments. Transplanting is generally performed one plant at a time using sterile tweezers. This method has several challenges: it is time-consuming, which makes analyzing short-term responses to nutritional changes difficult; plants can be mechanically damaged, which can confound results; transferring mature plants is accompanied by root wounding due to root penetration into the solidified medium; and root shapes are altered, which precludes a precise evaluation on nutritional responses of root system architecture. To solve these problems, this study tried to adopt a system for Arabidopsis culture using solidified media covered with cellophane disks for the transfer experiments to analyze nitrogen responses as a case study. The cellophane disk prevented the penetration of roots into the solidified medium and fixed the roots loosely; thereby, facilitating sterile, rapid and noninvasive transfer of young and mature Arabidopsis plants between media without changing root shape. The plants-on-cellophane grown on the solidified media were directly transferred onto hydroponics, where plants vegetate and propagate seeds under strict nutritional control. The root proliferation on the cellophane disk allowed direct observations of root architecture and green fluorescent protein (GFP) fluorescence in the roots. Collectively, we conclude that the introduction of a cellophane sheet to the existing culture system would accelerate experiments in plant nutritional research.

摘要

在不同营养介质之间转移植物是植物营养研究中的常用技术。固化的无菌培养基通常用于植物培养和随后的转移实验。通常使用无菌镊子一次移植一株植物。这种方法有几个挑战：耗时，难以分析对营养变化的短期反应；植物可能会受到机械损坏，这可能会混淆结果；由于根部渗透到凝固的培养基中，转移成熟植物伴随着根部受伤；根形状发生改变，这排除了对根系结构的营养反应的精确评估。为了解决这些问题，本研究尝试采用拟南芥系统使用覆盖有玻璃纸盘的固化培养基进行培养，以进行转移实验，以分析氮响应作为案例研究。玻璃纸盘防止根部渗入固化的介质，松散地固定根部；从而促进年轻和成熟拟南芥的无菌、快速和无创转移在不改变根形状的情况下在培养基之间种植。在固化培养基上生长的玻璃纸上的植物直接转移到水培上，在那里植物在严格的营养控制下生长和繁殖种子。玻璃纸盘上的根增殖允许直接观察根结构和根中的绿色荧光蛋白 (GFP) 荧光。总的来说，我们得出结论，将玻璃纸引入现有的培养系统将加速植物营养研究的实验。