The goals of this study were to evaluate growth, development, and calcification process of veligers of Strombus gigas grown in natural conditions in mesocosm versus laboratory conditions. In this study, larvae bred in mesocosm conditions had a good growth rate (33.3±12.40 μm.day⁻¹) when fed with natural phytoplankton in natural flowing seawater versus a lower growth rate of 8.8±5.20 μm.day⁻¹ for larvae reared in laboratory conditions and fed with a monoalgal diet of Nannochloropsis oculata. Physicochemical parameters did not explain the difference on larval growth in both culture systems according to the principal component analysis done. Raman microspectrometry carried out on conch larvae grown in mesocosm and lab conditions allowed us to emphasize the biosynthesis of calcium carbonate species and their structure type evolution (amorphous, aragonite, or calcite structure) as a function of the breeding time by detecting and identifying in the spectra the characteristic Raman bands of CO₃ chemical groups and lattice vibrations. This analytical method seems to indicate that crystalline CaCO₃ structures are not detected in the recorded spectra on larvae reared in laboratory conditions during the early stages of the shell construction of 1 to 8 days. For this reason, light and current flow have also been considered in the discussion that can help to explain the differences found in this study. Contrarily, the Raman spectra acquired on larvae grown in mesocosms exhibit characteristic bands of aragonite (CO₃ double peak at 697–701 cm⁻¹ and lattice vibrations at 170 and 220 cm⁻¹) from the third day of breeding. The best shell growth and shell calcification pointed out in larvae grown in mesocosm compared to laboratory conditions are probably due to the nutrient amounts present in the food available in mesocosm.

这项研究的目的是评估的生长，发育，和面盘幼虫的钙化过程凤牡蛎在围隔与实验室条件下的自然条件下生长。在这项研究中，在自然流动的海水中饲喂天然浮游植物时，在中观条件下繁殖的幼虫具有良好的生长速率（33.3±12.40μm.day ^-1），而在幼体中养殖的幼虫的生长速率较低，为8.8±5.20μm.day ^-1在实验室条件下并喂食Nannochloropsis oculata的单藻饮食。根据已完成的主成分分析，理化参数不能解释两种培养系统中幼虫生长的差异。在中膜和实验室条件下对海螺幼虫进行的拉曼光谱分析使我们能够通过检测和鉴定碳酸钙物种的生物合成及其作为繁殖时间的函数的结构类型演变（无定形，文石或方解石结构）。光谱分析了CO ₃化学基团的特征拉曼谱带和晶格振动。此分析方法似乎表明结晶的CaCO ₃在1到8天的外壳构造早期阶段，在实验室条件下饲养的幼体的记录光谱中未检测到结构。因此，讨论中还考虑了光流和电流，可以帮助解释本研究中发现的差异。相反，从繁殖的第三天开始，在中胚层中生长的幼虫上获得的拉曼光谱表现出文石的特征谱带（697-701 cm ^-1处的CO ₃双峰，以及170和220 cm ^-1处的晶格振动）。与实验室条件相比，在中层环境中生长的幼虫所指出的最佳壳生长和壳钙化可能是由于中层食物中存在的营养成分所致。