Pre-harvest sprouting is one of the most serious problems in rice production. Sensitive pre-harvest sprouting causes low seed quality and losses before harvesting. Seed dormancy is one of the most important traits relative to pre-harvest sprouting. In this study, the common wild rice, Oryza rufipogon W630 (strong seed dormancy) was used as a donor to improve the pre-harvest sprouting resistance of O. sativa Indica IR36 (popular cultivar in Asia). Thereafter, 170 backcross recombinant inbred lines (BRILs) at the BC₂F₈ generation were produced by the single seed descendant method. Their degree of dormancy was assessed and genotype was analyzed using 180 polymorphic SSR markers covering entire chromosomes. Finally, a QTL analysis was conducted by composite interval mapping to identify putative QTLs of seed dormancy and the wild allele effects to the background of typical rice cultivar IR36. Thus, two of 170 BRILs showed stronger seed dormancy compared to recurrent parent IR36. In addition, two putative QTLs for seed dormancy at chromosome 2 and 7 were also found in this population through composite interval mapping. These results indicated the stronger dormancy trait of two BRILs derived from wild rice Oryza rufipogon W630; this trait was also controlled by genetic factors.

收获前的发芽是水稻生产中最严重的问题之一。敏感的收获前发芽会导致种子质量低下，收获前损失少。相对于收获前发芽，种子休眠是最重要的性状之一。在这项研究中，使用普通野生稻Oryza rufipogon W630（强种子休眠）作为供体，以提高稻（O. sativa Indica）IR36（亚洲流行品种）的收获前发芽抗性。此后，在BC ₂ F ₈处有170个回交重组自交系（BRIL）一代是通过单种子后代方法产生的。使用覆盖整个染色体的180个多态性SSR标记评估了它们的休眠程度并分析了基因型。最后，通过复合区间作图法进行了QTL分析，以鉴定出种子休眠的假定QTL以及对典型水稻品种IR36的背景的野生等位基因效应。因此，与轮回亲本IR36相比，170个BRIL中有两个显示出更强的种子休眠性。此外，通过复合区间作图，在该种群中还发现了两个推定的2号和7号种子休眠的QTL。这些结果表明野生稻导出的两个BRILs的更强的休眠性状普通野生稻W630; 这种性状也受到遗传因素的控制。