A combined supercritical carbon dioxide and high power ultrasound treatment (SC-CO₂ + HPU) to inactivate spores while minimizing the physicochemical changes in emulsions was investigated. The inactivation kinetics were obtained for Bacillus subtilis, Bacillus pumilus and Geobacillus stearothermophilus spores and the effect of the treatment conditions on the quality of the emulsions was explored using response surface methodology. The treatment for 20 min at 95 °C and 350 bar was effective at inactivating B. subtilis and B. pumilus. Treated emulsions showed, in general, minimal changes in density and good stability, although the pH decreased and the droplet size increased. The treatment at 600 bar, 95 °C and 12.5 min permitted satisfactory B. subtilis and B. pumilus inactivation with minimal physicochemical changes in the emulsions. Therefore, the use of SC-CO₂ + HPU could be a viable alternative for the preservation of emulsions using temperatures lower than those of thermal treatments.

研究了超临界二氧化碳和高功率超声处理（SC-CO ₂ + HPU）的组合，可以灭活孢子，同时使乳剂的理化变化最小。获得了枯草芽孢杆菌，短小芽孢杆菌和嗜热脂肪芽孢杆菌孢子的失活动力学，并使用响应表面方法探讨了处理条件对乳状液质量的影响。在95°C和350 bar下处理20分钟对灭活枯草芽孢杆菌和短小芽孢杆菌有效。尽管pH降低且液滴尺寸增加，但处理过的乳液通常显示出最小的密度变化和良好的稳定性。在600 bar，95°C和12.5 min的条件下处理可使枯草芽孢杆菌和短小芽孢杆菌失活，并且乳液中的理化变化极小。因此，使用SC-CO ₂ + HPU可能是一种比使用热处理温度更低的温度来保存乳液的可行替代方法。