Mutations and single base pair polymorphisms in various genes have been associated with increased susceptibility to inflammatory bowel disease (IBD). We have created a series of rat strains carrying targeted genetic alterations within three IBD susceptibility genes: Nod2, Atg16l1, and Il23r, using CRISPR/Cas9 genome editing technology. Knock-out alleles and alleles with known human susceptibility polymorphisms were generated on three different genetic backgrounds: Fischer, Lewis and Sprague Dawley. The availability of these rat models will contribute to our understanding of the basic biological roles of these three genes as well as provide new potential IBD animal models.

各种基因中的突变和单碱基对多态性与炎症性肠病 (IBD) 的易感性增加有关。我们使用 CRISPR/Cas9 基因组编辑技术，在三个 IBD 易感基因：Nod2、Atg16l1和Il23r 中创建了一系列携带靶向遗传改变的大鼠品系。敲除等位基因和具有已知人类易感性多态性的等位基因是在三种不同的遗传背景下产生的：Fischer、Lewis 和 Sprague Dawley。这些大鼠模型的可用性将有助于我们理解这三个基因的基本生物学作用，并提供新的潜在 IBD 动物模型。