An eighty-day feeding study was performed to investigate the effects of mealworm meal (Tenebrio molitor) on the growth, survival and moulting frequency of juvenile Narrow-clawed crayfish (Pontastacus leptodactylus). Three dietary treatment groups with triplicate were fed with a fish meal-based without MW (control diet: MW0), two diets with 50% (MW50) and 100% (Mw100) of fish meal substitution with MW respectively. P. leptodactylus juveniles (11.0 ± 0.05 mm, mean ±SE and 0.011 ± 0.002 g, mean ±SE) were randomly stocked into nine flow-through tanks with a density of 30 crayfish per tank and reared for 80 days. Crayfish fed with MW50 mealworm meal showed positive effects on weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER), apparent net protein utilization (ANPU), moulting frequency (MF) and feed conservation ratio (FCR), but lower survival rate (SR) as compared to the other diet groups. Protein and lipid contents of the whole body of the crayfish fed with replacement diet (MW50) were found to be higher than those of the other two diet groups (p<0.05). However, the MW100 diet slightly increased the moisture and ash contents of the crayfish. Our observation indicated that the addition of a 50% mealworm meal in the diet of P. leptodactylus juveniles improved the growth performance of the crayfish without causing any adverse effects. Therefore, T. molitor meal can be considered as an alternative animal source of protein for the narrow-clawed crayfish juveniles.

中文翻译：

---

粉虫（黄粉虫）粉作为窄爪小龙虾（Pontastacus leptodactylus）幼鱼的替代蛋白质来源的评估

进行了一项为期八十天的饲养研究，以研究粉虫粉（黄粉虫）对窄爪小龙虾（Pontastacus leptodactylus）幼鱼的生长、存活和换羽频率的影响。三个一式三份的日粮处理组分别饲喂基于鱼粉的无 MW 鱼粉（对照日粮：MW0），两种日粮分别用 50% (MW50) 和 100% (Mw100) 的鱼粉替代鱼粉。P。钩爪鱼将幼虾（11.0±0.05mm，平均值±SE和0.011±0.002g，平均值±SE）随机放入9个流通池中，每池30只小龙虾，饲养80天。用 MW50 粉虫粉喂养的小龙虾对体重增加 (WG)、比生长率 (SGR)、蛋白质效率比 (PER)、表观净蛋白质利用率 (ANPU)、换羽频率 (MF) 和饲料保存率 (FCR) 显示出积极影响，但与其他饮食组相比，存活率 (SR) 较低。发现替代饮食（MW50）喂养的小龙虾全身的蛋白质和脂质含量高于其他两个饮食组（p<0.05）。然而，MW100 饮食略微增加了小龙虾的水分和灰分含量。我们的观察表明，在P的饮食中添加 50% 的粉虫粉。Leptodactylus juveniles 提高了小龙虾的生长性能，而不会造成任何不利影响。因此，T。墨鱼粉可被视为窄爪小龙虾幼体的替代蛋白质动物来源。