Nanoparticles (NPs) have properties for potential applications in different areas but their conventional production processes are often linked to secondary health and environmental impacts. Aggressive research endeavors are being undertaken to identify greener NPs synthesis strategies. Green synthesis is an evolving area with the objective of an efficient and environmental friendly development of NPs. Biological substances including bacteria, fungi, yeast, algae, and plant have been demonstrated to be applicable for the synthesis of TiO₂ NPs. This work is dedicated to phyto-based biosynthesis of TiO₂ NPs. Biomolecules in plants such as terpenoids, flavones, ketones, aldehydes, proteins, amino acids, vitamins, alkaloids, tannins, phenolics, saponins, and polysaccharides can play vital roles as a reducing, capping and stabilizing agent in the formation of TiO₂ NPs. A systemic comparison is made with the focus on the bioreduction ability of different plant extracts to produce specific TiO₂. Important results of applicable instrumentation techniques for characterizing the TiO₂ NPs are also elucidated to identify the superior qualities of the NPs. Moreover, photocatalytic and antimicrobial applications of the phytosynthesized TiO₂ NPs are also critically discussed. The main scopes are the current status of plant- assisted biosynthesis of TiO₂ NPs and future prospects of this promising area of research.

纳米颗粒（NPs）具有在不同领域中潜在应用的特性，但其常规生产过程通常与次生健康和环境影响相关。正在进行积极的研究努力，以识别更绿色的NP合成策略。绿色合成是一个不断发展的领域，其目标是高效，环保地开发NP。已经证明包括细菌，真菌，酵母，藻类和植物在内的生物物质可用于合成TiO ₂ NP。这项工作致力于TiO _2的植物基生物合成NP。诸如萜类化合物，黄酮，酮，醛，蛋白质，氨基酸，维生素，生物碱，单宁，酚类，皂角苷和多糖等植物中的生物分子在TiO ₂ NP的形成中起着还原，加帽和稳定剂的作用。系统地比较了不同植物提取物产生特定TiO ₂的生物还原能力。还阐明了用于表征TiO ₂ NP的适用仪器技术的重要结果，以确定NP的优良品质。此外，植物合成的TiO _2的光催化和抗菌应用NP也进行了严格的讨论。主要范围是TiO ₂ NP的植物辅助生物合成的现状以及这一有前途的研究领域的未来前景。