To understand the effects of the toxic dinoflagellate Alexandrium minutum on the marine copepod Tigriopus japonicus, we assessed acute toxicity and swimming behavior (swimming speed, swimming distance, and swimming path trajectory) in response to A. minutum exposure. No significant changes in survival and swimming behavior were observed in response to A. minutum. Therefore, to validate the effects of A. minutum on the molecular defense response of T. japonicus, we measured mRNA expression levels of detoxification-related genes (phase I cytochrome P450s [CYPs], and phase II glutathione-S transferases [GSTs]) in response to A. minutum. The mRNA expression levels of these detoxification-related genes (CYPs and GSTs) were significantly up-regulated and down-regulated (P < 0.05) in response to A. minutum. In particular, CYP3024A2 and GST-Theta3 showed significant up-regulation. Overall, these results suggest that CYP and GST genes are likely to play crucial roles in detoxification mechanisms in T. japonicus, resulting in no significant changes in the survival and swimming behavior of this marine copepod in response to A. minutum exposure.

为了了解有毒的鞭毛藻亚历山大藻对海洋co足类Tigriopus japonicus的影响，我们评估了急性毒性和游泳行为（游泳速度，游泳距离和游泳路径轨迹），以应对A. minutum暴露。没有观察到对A. minutum的存活和游泳行为有显着变化。因此，为验证min草对日本血吸虫分子防御反应的影响，我们测量了排毒相关基因（I期细胞色素P450 [ CYP s]和II期谷胱甘肽-S转移酶[ GST ]的mRNA表达水平。s]），以应对A. minutum。这些解毒相关基因的mRNA表达水平（CYP S和GST或多个）被显著上调和下调（P 响应于<0.05）A.藻。特别是，CYP3024A2和GST- Theta3表现出明显的上调。总体而言，这些结果表明，CYP和GST基因可能在日本血吸虫的解毒机制中起关键作用，从而导致该海洋co足类动物的存活和游泳行为没有显着变化，以应对微小min虫的暴露。