Agave bagasse is the lignocellulosic residue of the tequila industry and a potential feedstock for hydrogen production (H₂) via dark fermentation after proper saccharification. Highly specialized enzymes for hydrolysis of lignocellulosic biomass are costly, hindering their application in continuous processes. This work investigated the use of a generic cellulase (Cellulase 50XL), to prepare the hydrolysates from agave bagasse, either alone or combined with a highly specialized hemicellulase (Viscozyme L). The combination of Cellulase 50XL and Viscozyme L increased the release of both total carbohydrates and chemical oxygen demand (COD) in 28.5% and 22.2%, respectively compared with Cellulase 50XL. Conversely, the maximum volumetric H₂ production rate (VHPR, 9.9 L H₂/L-d) reached with the Cellulase 50 XL hydrolysates in continuous stirred tank reactors at an organic loading rate of 100 g COD/L-d, is the highest VHPR reported so far with enzymatic agave bagasse hydrolysates obtained with non-specialized enzyme. The estimated cost of H₂ production using Cellulase 50 XL at lab-scale was 0.7 USD/L H₂. From a systematic comparison of H₂ productivities and lab-scale costs with similar studies it was concluded that: i) the composition, and not only the concentration of total carbohydrates plays a critical role in the performance of H₂ production, ii) Cellulase 50 XL was one of the best cost-effective alternatives despite being designed for food sectors and not for the hydrolysis of lignocellulosic biomass. Overall, considering H₂ productivities and cost of H₂ production, Cellulase 50 XL hydrolysates resulted an appropriate option for cost-effective continuous H₂ production.

龙舌兰蔗渣是龙舌兰酒工业中的木质纤维素残留物，是经过适当糖化后通过黑暗发酵产生氢气（H ₂）的潜在原料。用于水解木质纤维素生物质的高度专业化的酶价格昂贵，阻碍了其在连续过程中的应用。这项工作研究了使用通用纤维素酶（Cellulase 50XL）从龙舌兰蔗渣中单独或与高度专业化的半纤维素酶（Viscozyme L）组合制备水解产物的方法。与纤维素酶50XL相比，纤维素酶50XL和Viscozyme L的组合分别增加了28.5％和22.2％的总碳水化合物释放量和化学需氧量（COD）。相反，最大体积H ₂生产率（VHPR，9.9  L H在连续搅拌釜反应器中，纤维素酶50 XL水解产物的有机负载率为100  g COD / Ld达到₂ / Ld），是迄今为止用非专业化酶获得的龙舌兰蔗糖酶水解产物的最高VHPR。在实验室规模使用Cellulase 50 XL生产H ₂的估计成本为0.7 USD / LH ₂。通过对H ₂生产率和实验室规模成本的系统比较以及类似研究得出的结论是：i）组成，不仅总碳水化合物的浓度在H ₂的性能中起关键作用ii）纤维素酶50 XL尽管是为食品行业设计的，而不是为木质纤维素生物质的水解而设计的，但它却是成本效益最好的替代品之一。总体而言，考虑ħ ₂生产率和H的成本₂生产，纤维素酶50 XL水解产物，导致成本效益的连续H成适当的选项₂的生产。