Abstract

A simple equation is proposed for calculating the sensitized luminescence kinetics of acceptors caused by nonradiative excitation energy transfer (Förster Resonance Energy Transfer (FRET)) from donors. As parameters, the equation includes the lifetimes of the donor and acceptor excited states and the experimental luminescence decay kinetics of donors in the presence of FRET. The equation does not specify a certain FRET mechanism. It is proposed to use this equation to fit the experimental sensitized luminescence kinetics in order to quantitatively confirm the existence of FRET between donor and acceptors.

中文翻译：

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敏化的发光动力学作为非辐射能量转移识别的工具

摘要

提出了一个简单的方程式，用于计算由供体的非辐射激发能量转移（福斯特共振能量转移（FRET））引起的受体的敏化发光动力学。作为参数，该方程包括供体和受体激发态的寿命以及在存在FRET的情况下供体的实验发光衰减动力学。该方程式未指定某种FRET机制。为了定量地确定供体和受体之间存在FRET，建议使用该方程式拟合实验的敏化发光动力学。