Chemiluminescence bioimaging with enhanced efficiency, ultra-low background, and reliable sensitivity are highly demanded. However, chemiluminescent sensors with substantial luminance efficiency, stability, and biocompatibility are quite rare. A novel activatable chemiluminescent biosensor (CL-N) for sensitively tracking hypochlorous acid (HOCl) in vivo is designed by incorporation of recognition ligand 4-aminophenyl ether with the adamantylidene-dioxetane system. The instant and efficient cleaving of the recognition group by trace HOCl has resulted in strong turn-on chemiluminescence with a detection limit of 10 nM. The chemiluminescence biosensor was successfully applied in chemiluminescence imaging of both endogenous and exogenous HOCl in RAW 264.7 cells and living mice in vivo. Using CL-N as an example, we have offered a versatile chemiluminescent platform with the vast potentiality for chemo/bioanalysis.

强烈要求化学发光生物成像具有更高的效率，超低背景和可靠的灵敏度。然而，具有相当高的发光效率，稳定性和生物相容性的化学发光传感器是非常罕见的。通过将识别配体4-氨基苯基醚与金刚烷-二氧杂环丁烷系统结合，设计了一种新颖的可激活的化学发光生物传感器（CL-N），用于在体内敏感地追踪次氯酸（HOCl）。痕量HOCl立即有效地裂解识别基团导致了强开启化学发光，检测限为10 nM。化学发光生物传感器已成功应用于RAW 264.7细胞和活小鼠中内源性和外源性HOCl的化学发光成像体内。以CL-N为例，我们提供了一种具有广泛化学/生物分析潜力的多功能化学发光平台。