Cell immobilization technology has shown much potential in the field of biological fermentation owing to its simple operation, maintenance of long-term cell viability, repeatability, good stability, and high tolerance. Compared with free-cell fermentation, immobilized cells show strong growth and metabolism performance in complex environments, such as environments with low pH, or with high concentrations of substrate and product. The metabolic properties of immobilized cells mainly depend on the carrier materials. In recent years, polymer membrane materials have attracted much attention because of their good mechanical properties and flexible support structure. In addition to improving the activity and stability of immobilized cells, biocompatibility modification can also be carried out on the surface of the carrier, which can reduce specific non-biological interactions between the cells and the carrier. In this review, current advances in cell immobilization using different materials in the field of biological fermentation are reviewed. Perspectives on immobilized cells and the challenges associated with them in the field of biological fermentation are also addressed. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

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生物发酵过程中材料介导的细胞固定化技术

细胞固定化技术因其操作简单、保持细胞长期活力、重复性好、稳定性好、耐受性高等优点，在生物发酵领域显示出巨大的潜力。与游离细胞发酵相比，固定化细胞在复杂环境中表现出较强的生长和代谢性能，如低pH环境或高浓度底物和产物。固定化细胞的代谢特性主要取决于载体材料。近年来，高分子膜材料因其良好的机械性能和灵活的支撑结构而备受关注。除了提高固定化细胞的活性和稳定性外，还可以在载体表面进行生物相容性修饰，这可以减少细胞和载体之间的特定非生物相互作用。在这篇综述中，回顾了生物发酵领域使用不​​同材料进行细胞固定的最新进展。还讨论了固定化细胞的前景以及与它们在生物发酵领域相关的挑战。© 2021 化学工业协会和 John Wiley & Sons, Ltd