The measurement of steroid hormones provided critical information in the clinical evaluation of endocrine disorders. In this study, we developed a high-throughput solid-phase extraction method for the analysis of 26 steroids in human serum and plasma samples by liquid chromatography–tandem mass spectrometry. Chromatographic conditions and sample preparation were optimized to achieve good separation and maximum sensitivity for these analytes. Under the optimum conditions, good linearities were achieved in the quantitative range for each steroid hormone with the correlation coefficients (r) larger than 0.99. The limits of quantitation of the method were in the range from 0.0005 to 0.7901 ng/mL. The recoveries were in the range of 87.2–114.2% with intra- and interday precision lower than 9.94%. This method has already been applied to series of samples from clinical trials, and there was no significant difference between serum and ethylenediaminetetraacetic acid plasma samples.

中文翻译：

---

人血清和血浆样品中 26 种类固醇激素的液相色谱-串联质谱测量

类固醇激素的测量为内分泌疾病的临床评估提供了关键信息。在本研究中，我们开发了一种高通量固相萃取方法，用于通过液相色谱-串联质谱法分析人血清和血浆样品中的 26 种类固醇。对色谱条件和样品制备进行了优化，以实现对这些分析物的良好分离和最大灵敏度。在最佳条件下，每种类固醇激素在定量范围内都获得了良好的线性，相关系数为 ( r) 大于 0.99。该方法的定量限在 0.0005 到 0.7901 ng/mL 的范围内。回收率在 87.2-114.2% 的范围内，日内和日间精密度低于 9.94%。该方法已应用于临床试验的系列样本，血清和乙二胺四乙酸血浆样本无显着差异。