2,5-Furandicarboxylic acid (FDCA) is a valuable compound that can be synthesized from biomass-derived hydroxymethylfurfural (HMF), and holds great potential as a promising replacement for petroleum-based terephthalic acid in the production of polyamides, polyesters, and polyurethanes used universally. However, an economical large-scale production strategy for HMF from lignocellulosic biomass is yet to be established. This study aimed to design a synthetic pathway that can yield FDCA from furfural, whose industrial production from lignocellulosic biomass has already been established. This artificial pathway consists of an oxidase and a prenylated flavin mononucleotide (prFMN)-dependent reversible decarboxylase, catalyzing furfural oxidation and carboxylation of 2-furoic acid, respectively. The prFMN-dependent reversible decarboxylase was identified in an isolated strain, Paraburkholderia fungorum KK1, whereas an HMF oxidase from Methylovorus sp. MP688 exhibited furfural oxidation activity and was used as a furfural oxidase. Using Escherichia coli cells coexpressing these proteins, as well as a flavin prenyltransferase, FDCA could be produced from furfural via 2-furoic acid in one pot.

2,5-呋喃二甲酸 (FDCA) 是一种有价值的化合物，可以由生物质衍生的羟甲基糠醛 (HMF) 合成，在聚酰胺、聚酯和聚氨酯的生产中作为石油基对苯二甲酸的有前途的替代品具有巨大的潜力普遍使用。然而，尚未建立从木质纤维素生物质中生产 HMF 的经济大规模生产策略。本研究旨在设计一种可以从糠醛中产生 FDCA 的合成途径，其从木质纤维素生物质的工业生产已经建立。该人工途径由氧化酶和异戊二烯化黄素单核苷酸 (prFMN) 依赖性可逆脱羧酶组成，分别催化糠醛氧化和 2-糠酸羧化。Paraburkholderia fungorum KK1，而来自Methylovorus sp.的 HMF 氧化酶。MP688 表现出糠醛氧化活性并用作糠醛氧化酶。使用共表达这些蛋白质的大肠杆菌细胞以及黄素异戊二烯基转移酶，可以在一锅中通过 2-糠酸从糠醛生产 FDCA。